Background: The honey bee of the Apis mellifera species is main type cultivated in almost all countries world, including Indonesia. Honey has benefits various aspects, food, health, and beauty. Royal jelly a daily food menu as supplement to maintain increase health vitality. Purpose: Analyzing immunogenic toxinogenic proteins carpatica immunotherapy. Method: Conversion nucleotides into amino ac...

The role of epitope expression levels in CD8+ T cell priming has been controversial. Yet this parameter is of great importance in the design of rational approaches to optimize CTL responses to a variety of pathogens. In this paper we examine the influence of epitope production on CD8+ T cell priming by exploiting a system that allows a 200-fold range of cell surface epitope expression in vitro ...

Varicella-zoster virus (VZV) glycoprotein H (gH) is the major neutralization target of VZV, and its neutralizing epitope is conformational. Ten neutralizing human monoclonal antibodies to gH were used to map the epitopes by immunohistochemical analysis and were categorized into seven epitope groups. The combinational neutralization efficacy of two epitope groups was not synergistic. Each epitop...

In this paper, informational (Shannon) measures of symmetry are introduced and analyzed for patterns built 1D 2D shapes. The measure Hsym(G) characterizes the averaged uncertainty in presence elements from group G a given pattern, whereas Shannon-like Ωsym(G) quantifies appearance shapes possessing total n belonging to pattern. Hsym(G1)=Ωsym(G1)=0 irregular, non-symmetric shapes, where G1 is id...

We previously demonstrated that the C-terminus of the capsid gene of porcine circovirus type 2 (PCV2) is an immune reactive epitope displayed on the surface of virions. Insertion of foreign epitope tags in the C-terminus produced infectious virions that elicited humoral immune responses against both PCV2 capsid and the inserted epitope tags, whereas mutation in the N terminus impaired viral rep...

It has been recently reported by our group that dendrimeric constructs combining B- and T-cell epitopes from classical swine fever virus (CSFV) provided partial protection against experimental infection. This research evaluated four newly designed constructions while taking into account our previous work, including the direct implication that a T-cell epitope from the NS3 protein contributes to...

Let G1 and G2 be two graphs on disjoint sets of n1 and n2 vertices, respectively. The corona of graphs G1 and G2, denoted by G1 ◦ G2, is the graph formed from one copy of G1 and n1 copies of G2 where the i-th vertex of G1 is adjacent to every vertex in the i-th copy of G2. The neighborhood corona of G1 and G2, denoted by G1 ⋄ G2, is the graph obtained by taking one copy of G1 and n1 copies of G...

B13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to La...

With the advent of class II tetramer technology, a tetramer-guided epitope mapping (TGEM) technique was developed for the identification of CD4+ T cell epitopes. This allowed the direct identification of epitopes recognized by the responding T cells, which were restricted to the single MHC allele of interest. However, as each individual carries multiple class II alleles, it would be advantageou...