A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a metha...

The development of various drugs from medicinal plants are based on its phytochemical and pharmacological approaches for curing different diseases, therefore, recognized in the pharmaceutical industries their broad structural diversity wide range activities. These phytochemicals compounds used as sources direct agents or can serve a raw material base elaboration more complex semi-synthetic chem...

Human lung type II cell derived A549 epithelial cancer cells and HepG2 hepatocytes constitutively express cytochrome P4504F2, a P450 we previously identified as a tocopherol-omega-hydroxylase. To determine if A549 cells would metabolize tocochromanols via the omega-hydroxylase pathway, we compared the metabolism of tocopherols (alpha-, gamma-, delta-TOH) and tocotrienols (alpha-, gamma-, delta-...

noise induced hearing loss (nihl) is one of the most important occupational disease worldwide. nihl has been found potentiate by simultaneous carbon monoxide (co) exposure. free radicals have been implicated in cochlear damage resulted from the exposure to noise and due to the co hypoxia. this study examined whether α-tocopherol administration, as a free radical scavenger, causes the attenuatio...

Tocopherols extracted from plasma with methanol or from platelets with chloroform/methanol were injected in methanol on a reversed-phase (C18) "high-performance" liquid-chromatographic column and eluted with water/methanol (2/98, by vol) at a flow rate of 1.4 mL/min. A "high-performance" spectrophotofluorometer was used for detection. Analytical recoveries ranged from 89 to 106%. The response w...

We have adapted to erythrocytes a method for the determination of alpha- and gamma-tocopherols in plasma and platelets. Erythrocytes (50 microL) were extracted with methanol containing tocol (internal standard) and pyrogallol. Tocopherols were partitioned into chloroform, washed, and injected in methanol onto a reversed-phase (C18) "high-performance" liquid-chromatographic column. The mobile ph...

Given that oxidative stress is an inherent response to burn injury, it is puzzling as to why investigation into anti-oxidant therapy as an adjunct to burn treatment has been limited. Both the inflammatory response and the stress response to burn injury involve oxidative stress, and there has been some limited success in studies using gamma tocopherol and selenium to improve certain consequences...

The objective of this study was to evaluate the dynamics of tocopherols in cotyledons and radicles from sunflower seeds with high and low total tocopherol content, mainly in the α-tocopherol form, and from seeds with increased proportions of β-, γ-, and δ-tocopherol, both under dark and light conditions. Tocopherol content was measured every 24 h from 1 to 12 days after sowing. In all cases, th...

we studied the effect of different concentrations of alpha-tocopherol on in vitro cupric ions induced oxidation of low density lipoproteins (ldl). human native ldl (50 µg protein/ml) oxidation was induced by 10 µmol/l of cuso4. conjugated dienes were measured spectrophotometrically for up to 440 minutes. the length of the lag phase (tlag), maximum velocity of the reaction (vmax) and the maximum...