Karet alam terdeproteinasi (DPNR) diproduksi untuk memenuhi kebutuhan persyaratan produk gangguan alergi protein dan proses modifikasi partikel karet alam. DPNR dibuat dengan teknik plasma solusi pengaplikasian beberapa nilai voltase yaitu 1, 5, 10 kV mengamati degradasi tertinggi bagian non-karet di DPNR. yang dihasilkan melalui tegangan selanjutnya disebut 1 kV, 5 kV. Degradasi ditentukan men...

The homogenate was subjected to centrifugation at 15 OOOg,, for IOmin and the residue was then washed with buffer A and re-centrifuged. The combined supernatant fractions were centrifuged at 23000g,,. for 60min and then stirred for 20min with DEAE-cellulose ( IOOml settled volume) that had been equilibrated in buffer A. The slurry was then poured into a column (lOx4cm) and washed with buffer A ...

Secondary metabolites from herbal are commonly used to treat disease due infection pathogenic bacteria, endophytic bacteria is shortcut collect secondary more effective than extracting direct the plant. This research aims obtain bacterial isolates stems of red betel plant which can be as an inhibitor for growth bacteria. The method this exploratory descriptive experiment based research. stages ...

In this work, the effect of KBrO3 on photodegradation mechanism Nb-TiO2 nanocomposites was analyzed. The photocatalytic activities were evaluated by using a high concentration phenol red (PR). fabricated simple sol–gel route with new experimental conditions. HRTEM and EDX used to study structural properties nanocomposites. decreased degradation time 20 mg·L?1 110 min, shorter than that in our p...

Abstract Bacterial conjunctivitis characterized as pink eye referred as an inflammation of an eye caused by the enlargement of blood vessels present in conjunctiva, resulting in a red or bloodshot appearance of the eyes. Topical ocular delivery is found to be useful in treating conjunctivitis, but to maintain an effective drug concentration at a site of action in order to achieve desired pha...

In this study, we describe the experiments determining whether coating gold nanoparticles with tetraethylene glycol (TEG) provides pharmacologically relevant advantages, such as increased serum half-life and resistance to protein adsorption. Monodisperse TEG-coated, NaBH4-reduced gold nanoparticles with a hydrodynamic size comparable to albumin were synthesized by reducing gold chloride with Na...

1. Galactokinase from normal human red blood cells was purified 3300to 3900-fold by ion exchange chromatography, ammonium sulfate precipitation, and dextran gel filtration with a yield of 14 to 19 %. Analytical disc electrophoresis of the purified enzyme revealed a single protein band. 2. The Km of purified galactokinase for galactose is 100 to 150 PM; for ATP, 200 to 500 PM. The pH optimum for...

Sera obtained from senescence-accelerated mouse (SAM) and normal mice contained a substance that reacted with antiserum raised against ASSAM, a novel senile amyloid fibril protein isolated from the liver of SAM. This physiological substance, termed "SASSAM" (serum ASSAM-related antigenic substance), migrated to the albumin/prealbumin region in immunoelectrophoresis and the precipitation line fo...

A type of ligninolytic enzyme was produced in the culture supernatant of Fomitopsis pinicola mycelia. The laccase was purified by ion-exchange chromatography on DEAE-Sepharose FF, gel filtration chromatography on Superdex 200 pg, and HIC chromatography on Phenyl FF from the culture supernatant of F. pinicola. The molecular weight of the enzyme was 92 kDa as determined by SDS-PAGE and gel filtra...

The fatty acid:CoA ligase (acyl-CoA synthetase, EC 6.2.1.3) of rat liver microsomes was solubilized with Triton X-100 and bound to Matrex Gel Red A. Fatty acid:CoA ligase immobilized on Matrex Gel Red A was active and proved useful for the synthesis of fatty acyl-CoA thioesters. The immobilized activity was characterized by a 3-fold higher apparent Km for ATP than the soluble activity, similar ...