نتایج جستجو برای: gene assembling
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Plasmids construction Codons of human Pou5f1/Oct4 (NP_002692), Sox2 (NP_003097), Klf4 (NP_004226), and cMyc (NP_002458) were first optimized for high level protein expression in E. coli. They were then synthesized using DNA oligo based, PCR gene assembling method. Poly-arginine tag plus a linker sequence, i.e. ESGGGGSPGRRRRRRRRRRR, was added to each protein C-terminal. The final DNA fragment wa...
There are two key challenges to creating the next generation applications: assembly and differentiation. Robust, inexpensive assembly is required at the very lowest levels (assembling nanoscale low-power economical computing devices) and the highest levels (assembling distributed applications from reusable low-cost software components). Differentiation is less obvious, but in some sense a more ...
Alternanthera philoxeroides is a notorious invasive weed worldwide, but it still lacks genome information currently. In this study, we collected 4 groups of A. Illumina RNA-seq data (62.5 Gb) and performed comprehensive de novo assembling. Totally, 421,372 unigenes were obtained with total length 230,842,460 bp, 43,430 (10.31%) longer than 1000 bp. Then 119,222 (28.3%) functional annotated 235,...
Nanoparticle-based therapeutics have been applied in a broad range of clinical and preclinical applications from diagnosis to treatment for cancer. A wide synthetic naturally occurring materials, such as polymers, metal oxides, silicate, liposomes, carbon nanotubes, developed overcome key barriers small molecule therapeutics, including intracellular trafficking, cell/tissue targeting, poor biod...
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