نتایج جستجو برای: gene transfer techniques
تعداد نتایج: 1962942 فیلتر نتایج به سال:
objective(s) recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. in the present experiment, a reco...
The remarkable advances in our understanding of the molecular and biochemical bases for disease during the past decade, in conjunction with the “genomic revolution,” have generated understandable enthusiasm for the development of genetic therapies. Whereas the initial hope was that gene therapy would aid in the treatment of patients with primary genetic disorders, applications have now been exp...
Gene therapy is emerging as a potential strategy for the treatment of cardiovascular disease, such as restenosis after angioplasty, vascular bypass graft occlusion, and transplant coronary vasculopathy, for which no known effective therapy exists. The first federally approved human gene therapy protocol started on September 14, 1990, in adenosine deaminase–deficienct patients. Eight years since...
Insights concerning leukemic pathophysiology have been acquired in various animal models and further efforts to understand the mechanisms underlying leukemic treatment resistance and disease relapse promise to improve therapeutic strategies. The zebrafish (Danio rerio) is a vertebrate organism with a conserved hematopoietic program and unique experimental strengths suiting it for the investigat...
Heat exchangers are widely used in power engineering and industrial applications, many techniques such as coiled tube, surface tension devices, rough surfaces, extended surfaces have been investigated to enhancethermal performance and to minimize the cost and size of the heat exchanger equipment. One of the most important techniques is tube insert. In general,tube inserts can be classified into...
Gene therapy has developed during the last two decades as a promising strategy for orthopaedics applications, since several different gene transfer techniques proved to be effective, both in vitro and in vivo, for the induction of bone formation. Successful results have been achieved with gene-based bone healing strategies in several preclinical studies, using different animal models. New genes...
Genetic modification of hematopoietic stem cells with genes that inhibit replication of human immunodeficiency virus-1 (HIV-1) could lead to development of T lymphocytes and monocytic cells resistant to HIV-1 infection after transplantation. We performed a clinical trial to evaluate the safety and feasibility of this procedure, using bone marrow from four HIV-1-infected pediatric subjects (ages...
In Vivo RNAi Rescue in Drosophila melanogaster with Genomic Transgenes from Drosophila pseudoobscura
BACKGROUND Systematic, large-scale RNA interference (RNAi) approaches are very valuable to systematically investigate biological processes in cell culture or in tissues of organisms such as Drosophila. A notorious pitfall of all RNAi technologies are potential false positives caused by unspecific knock-down of genes other than the intended target gene. The ultimate proof for RNAi specificity is...
Abstract Among synthetic carriers, dendrimers with the more flexible structure have attracted a great deal of researchers’ attention in the field of gene delivery. Followed by the promising results upon hydrophobic modification on polymeric structures in our laboratory, alkylcarboxylated poly (propylenimine)-based carriers were synthesized by nucleophilic substitution of amines with alkyl moiet...
We present new vibrational (infrared) planar laser-induced fluorescence (PLIF) imaging techniques for CO(2) that use a simple, inexpensive, high-pulse-energy transversely excited atmospheric CO(2) laser to saturate a CO(2) absorption transition at 10.6 mum. Strong excitation by use of a CO(2) laser provides increased signal levels at flame temperatures and simplifies image interpretation. Becau...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید