This study was aimed to rapidly identify and differentiate two main pathogens of the Mycobacterium tuberculosis complex: Mycobacterium tuberculosis subsp. tuberculosis and Mycobacterium bovis by a modified loop-mediated isothermal amplification (LAMP) assay. The reaction results could be evaluated by naked eye with two optimized closed tube detection methods as follows: ...

The cross-immunity between tuberculosis and leprosy is unknown. The aim of this pilot study was to evaluate the occurrence of Mycobacterium tuberculosis and M. leprae infection in Marshallese adult volunteers in Springdale, Arkansas, U.S.A., a population that experiences high rates of leprosy and tuberculosis. We used immunodiagnostic testing for tuberculosis and leprosy infection and found sig...

Tuberculous infection of the female genitalia flight occur either as a primary affection of the local site, or as a part of a general infection, the original lesion being in some other organ. Primary genital tuberculosis israre, the commonestcause being secondary infection of the genital tract, either through the blood, lymphatics, or the peritoneum. Hence incidence of genital tuberculosis in t...

Background: Cutaneous tuberculosis (TB) is a chronic infectious disease affecting the skin caused by Mycobacterium (M. tuberculosis). It constitutes 1–1.5% of extrapulmonary TB, with Indonesia being third-highest TB burden country worldwide. difficult to diagnose due its varied presentations. This study aimed describe clinicodemographic and laboratory characteristics cutaneous in tertiary refer...

In a search for new skin test reagents specific for tuberculosis, we found that the antigen encoded by gene Rv3874 of Mycobacterium tuberculosis elicited delayed-type hypersensitivity in M. tuberculosis-infected guinea pigs but not in control animals immunized with Mycobacterium bovis bacillus Calmette-Guérin (BCG) or Mycobacterium avium. The antigen, which was named MTSA-10 (for M. tuberculosi...

Development of new tuberculosis vaccine will be an important strategy to eliminate tuberculosis in kwashiorkor. Previous research showed that 38-kDa Mycobacterium tuberculosis protein is one of the potent immunogen. However, the role of oral immunization with 38kDa Mycobacterium tuberculosis protein to the number of lymphocytes in the rat model of kwashiorkor is still unknown. We used kwashiork...

In spite of the great advancement in the diagnosis, treatment and control programs of tuberculosis during the past decades, bovine tuberculosis still represent an imminent threat to the public and considerable obstacles for development of bovine industry especially in developing countries [1]. All animal species and humans of all age groups are susceptible to the disease. Mycobacterium bovis, a...

conclusions: pcr method is a valuable, cost-effective and alternative tool for quick diagnosis of active tuberculosis in different clinical specimens. results: the concordance rate between the three sets of primers was calculated and is6110/buffer pcr method showed good agreement with the lj culture method (κ = 0.627, p < 0.0001). the sensitivity of is6110/buffer pcr was 58.33%, with specificit...

conclusions a high index of suspicion is required for the early diagnosis and prompt treatment of patients with osseous tuberculosis. given the high prevalence of tuberculosis in developing countries, tuberculosis should be considered in the differential diagnosis of multifocal lytic lesions and vertebra plana, especially in children. introduction multifocal skeletal tuberculosis is a rare cond...