Australian spiders of the genus Abstract. -Three species of the spider genus Physocyclus (P. calijbrnicus, P. enaulus. P, sp.) . SOC. 96:359-362. were karyotyped using an air-drying, Giemsa staining method. All chromosomes were metacentric, with males being 2n = 15 (N = 7 + XO) and females being 2n = 16. The karyotyping method resulted in slide preparations which were countable five years post-...

A sequential silver-Giemsa (SG) procedure is presented, initially to stain the p11 and q11 euchromatic bands and subsequently the q12 heterochromatic band of the human Y chromosomes. A three sub-band division of the q11 band can be identified. The same technique differentially stains the secondary constriction of chromosome 9 as well as most other satellite III DNA regions of the human karyotype.

This paper describes the overnight Giemsa staining method for detecting mycoplasma infection of cell cultures. This simple method requires no special equipment and has practical application in most cell culture laboratories. This technique has been used conveniently to monitor stock cell cultures for mycoplasma infection over a 12-month period avoiding any contamination.