R apid and accurate diagnosis is a key to the success of an effective treatment of malaria. The global impact of malaria has spurred interest in developing diagnostic strategies that will be effective not only in resource-limited areas but also in developed countries, where expertise in malaria diagnosis is often lacking. Accurate diagnosis of malaria is necessary to prevent morbidity and morta...

A fluorescein isothiocyanate-labeled monoclonal cytomegalovirus antibody reagent incorporating Evans blue (Syva/Genetic Systems, Palo Alto, Calif.) was compared to a fluorescein isothiocyanate-labeled polyclonal antibody (Dynatech Diagnostics, Inc., South Windham, Maine) for identification of cytomegalovirus in tissue culture. A total of 191 cultures, 86 positive and 105 negative for cytomegalo...

Hemocytin, a multidomain protein from Bombyx mori L. (Lepidoptera: Bombycidae), is an ortholog of von Willebrand factor and is expected to be a major mediator of hemocyte aggregation. Antiserum was generated against hemocytin, and immune staining of hemocytes, hemolymph, and nodules was performed. Hemocytin was observed in steady-state hemocytes but not in plasma, even after bacterial injection...

Gastric biopsies are often done to evaluate for Helicobacter gastritis. Given the oncogenic association with Helicobacter gastritis and the relative ease of therapy, it is important for pathology departments to identify all positive cases. We describe an institutional quality assurance study of an institutional method for the diagnosis of Helicobacter gastritis. We reviewed 356 gastric biopsy s...

The detection of prostate-specific antigen (PSA) and visualization of spermatozoa from forensic-type samples containing semen exposed to simulated gastric juice was investigated as a support for forensic practice. Samples of simulated gastric juice mixed with semen were prepared and incubated for up to 4 h at 37 degrees C. Samples were deposited on cotton cloth and on ceramic plates and allowed...

The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fragments of active CL lesions were examined as well as 10 fragments of cutaneous mycosis lesions a...

In order to establish a method usable in the field for diagnosis of pre-clinical vitamin A deficiency, conjunctival impression cytology with transfer (CICT) was used in 200 normal slum children aged 6-120 months in Chandigarh, India. Conjunctival impressions taken on cellulose acetate paper were transferred to glass slides which were fixed in ethanol and stained with alcian blue and carbol fuch...