A monoclonal antibody, 4G10.3, was developed that preferentially binds limbal basal cells in adult rat, rabbit, and human corneas. These cells were hypothesized to be the stem cells for the corneal epithelium. The antibody 4G10.3 was localized by immunofluorescence microscopy in rats 1 d and 1, 1.5, 2, 3, 4, and 6 wk of age. Until 1.5 wk, 4G10.3 bound intensely to all basal cells in the cornea ...

the objective of this research was to investigate the efficacy of dietary inclusion of probiotics primalac® and bactocell® and prebiotic fermacto® on broiler’s performance and immune response, individually or in combination. a total of 540 one-d-old male ross 308 broiler chicks were allocated into 6 experimental treatments with 6 replicates of 15 birds per replicate from 1 to 42 d of age. the b...

Epithelial cells within the normal breast duct seem to be the primary target for neoplastic transformation events that eventually produce breast cancer. Normal epithelial cells are easily isolated and propagated using standard techniques. However, these techniques almost invariably result in populations of cells that are largely basal in character. Because only approximately 20% of human breast...

Epithelial cells within the normal breast duct seem to be the primary target for neoplastic transformation events that eventually produce breast cancer. Normal epithelial cells are easily isolated and propagated using standard techniques. However, these techniques almost invariably result in populations of cells that are largely basal in character. Because only f20% of human breast cancers exhi...

The epithelia lining the epididymides of many species consists of several cell types. We have provided evidence that the basal cells are essential to the integrated functions of the epithelium. Basal cells, but not principal cells, and other cells in the epididymis express TRPC3 and COX-1. We have isolated basal cells from intact rat epididymis using antibody-coated Dynabeads and subjected them...

Aloe vera is well-known for its pharmacological and nutritional properties. The aim of the present study was to determine the effect of A. vera gel extracts on the secretion and cell content of triglyceride (TG) and cholesterol (TC) in HepG2 cells and their short-term effects on the dietary hyperlipidemic guinea pig model. The effects of increasing concentrations of A. vera crude gel and its al...

This experiment was conducted to determine the effects of Myrtle Essential Oil (MEO) on growth performance, carcass characteristics, antibody titer and blood parameters of broiler chickens. A total of 200 Ross 308 broiler chickens were allocated to five dietary treatments with four replicates of 10 birds each. Dietary treatments were prepared by formulating a corn-soybean meal-based diet free o...

In the current study, laser scanning confocal image analysis was used to investigate the actin distribution in whole-mount preparations of freshly isolated and cultured corneal epithelia. Actin staining defined the cell borders and microvilli of the periderm cells. The actin was prominent as an organized network at the interface between the basal and periderm cells and in the basal compartment ...