The effect of dehydration on the specific activities and transcript levels of several glycolytic enzymes was determined in two chickpea (Cicer arietinum L.) cultivarsPusa362 (drought tolerant) and SBD377 (drought sensitive). Dehydration treatment was imposed on 6 days old chickpea seedlings by removing them from pots and keeping them on table top on 3MM Whatman paper at room temperature for 5 h...

Regulation of glucose metabolism is a crucial aspect of cell physiology in normal and disease conditions. Many regulatory events are involved in determining the metabolic fate of glucose and the pathways into which it is directed. The first reaction that commits glucose to the glycolytic pathway is catalyzed by the enzyme phosphofructokinase-1 (PFK-1) and is tightly regulated. One of the most p...

Neutrophils release free zinc to eliminate the phagocytosed bacterial pathogen Streptococcus pyogenes (Group A Streptococcus; GAS). In this study, we investigated the mechanisms underpinning zinc toxicity towards this human pathogen, responsible for diseases ranging from pharyngitis and impetigo, to severe invasive infections. Using the globally-disseminated M1T1 GAS strain, we demonstrate that...

Although differences among species in enzyme maximal activity or concentration are often interpreted as adaptive and important for regulating metabolism, these differences may simply reflect phylogenetic divergence. Phylogenetic analysis of the expression of the glycolytic enzymes among 15 taxa of a North American fish genus (Fundulus) indicated that most variation in enzyme concentration is du...

It is now well established that most animal tissues contain enzymes of a non-glycolytic pathway, the pentose phosphate pathway, in addition to those involved in anaerobic glycolysis (1, 2). Skeletal muscle, however, appears to contain glucose--phosphate dehydrogenase and 6-phosphogluconic dehydrogenase, enzymes of this alternate pathway, in relatively low concentrations. The relative importance...

Glycolysis is the core metabolic pathway supplying energy to cells. Whereas the vast majority of studies focus on specific aspects of the process, global analyses characterizing simultaneously all enzymes involved in the process are scarce. Here, we demonstrate that quantitative label- and standard-free proteomics allows accurate determination of titers of metabolic enzymes and enables simultan...

We used beta-escin-permeabilized pig cerebral microvessels (PCMV) to study the organization of carbohydrate metabolism in the cytoplasm of vascular smooth muscle (VSM) cells. We have previously demonstrated (Lloyd PG and Hardin CD. Am J Physiol Cell Physiol 277: C1250-C1262, 1999) that intact PCMV metabolize the glycolytic intermediate [1-(13)C]fructose 1,6-bisphosphate (FBP) to [1-(13)C]glucos...

-The intracellular distribution and maximal activities of phosphorylase, trehalase, all the enzymes of glycolysis, NAD and FAD-linked glycerol 3-phosphate dehydrogenase, arginine kinase, and adenylate kinase have been determined in the fat body of the adult male cockroach Periplaneta americana. Phosphorylase activity is predominantly in the soluble form but some can be sedimented. Both forms of...

We have prepared a functional fluorescent analogue of the glycolytic enzyme aldolase (rhodamine [Rh]-aldolase), using the succinimidyl ester of carboxytetramethyl-rhodamine. Fluorescence redistribution after photobleaching measurements of the diffusion coefficient of Rh-aldolase in aqueous solutions gave a value of 4.7 × 10 -7 cm2/s, and no immobile fraction. In the presence of filamentous acti...

The activity of chymosin, plasmin, and Lactococcus lactis enzymes (cell envelope proteinase, intracellular peptidases, and glycolytic enzymes) were determined after 5-min exposures to pressures up to 800 MPa. Plasmin was unaffected by any pressure treatment. Chymosin activity was unaffected up to 400 MPa and decreased at 500 to 800 MPa. Fifty percent of control chymosin activity remained after ...