Results: Out of a total of 1970 specimens, 1094 (55.5 %) were positive by culture. A total of 1462 strains were isolated: 753 Gram positive (Gram+), 661 Gram negative (Gram-) and 48 anaerobic bacteria. The number of specimens yielding one, two or more different strains was 788, 244 and 62, respectively. Gram + bacteria, in 44.7 % of positive samples were a single isolate. The most commonly isol...

Several species of anaerobic bacteria display variable Gram stain reactions which often make identification difficult. A simple, rapid method utilizing a 3% solution of potassium hydroxide to distinguish between gram-positive and gram-negative bacterial was tested on 213 strains of anaerobic bacteria representing 19 genera. The Gram stain reaction and KOH test results were compared with the ant...

Sclerorhachis platyrachis essential oil: Antioxidant power, total phenolic and flavonoid content its antimicrobial activity on some Gram-positive Gram-negative bacteria “in vitro”

a field experiment was conducted at research station of zabol university in 2012. the experiment carried out as split plot on basis of a randomized complete block design with three replications. the main and sub factors included tillage systems (no, reduced, and conventional tillage) and planting ratios of intercropping (pure culture of roselle, pure culture of green gram, 25% roselle + 75% gre...

The increasing prevalence of multiresistant Gram-negative bacteria of the Enterobacteriaceae family in Europe is a worrisome phenomenon. Extended spectrum betalactamase-producing Escherichia coli strains are widespread in the community and are frequently imported into the hospital. Of even more concern is the spread of carbapenem-resistant strains of Klebsiella spp. from regions where they are ...

Several papers have been published (Kennedy and Barbaro, 1953; Barbaro and Kennedy, 1954; Bartholomew and Finkelstein, 1954; Kennedy and Woodhour, 1956; Barbaro et al., 1956) on methods of comparing degrees of gram-positiveness and the influence of certain factors on the quantity of crystal violet retained by the microorganisms. These papers compare the degrees of gram-positiveness by measuring...

To cite: Hadano Y. BMJ Case Reports Published online: [please include Day Month Year] doi:10.1136/ bcr-2012-008477 DESCRIPTION A 65-year-old Japanese man with advanced lung cancer was admitted with a 2-week history of cough and general fatigue. On physical examination, the patient appeared to be chronically ill; his blood pressure was 115/52 mmHg, pulse rate was 97 beats/min and regular, temper...

At the present time there is no satisfactory procedure for estimating the degree of gram positivity although the need for a quantitative method is widely recognized. Recently Kennedy and Barbaro (1953) reported an accurate and reproducible method for determining quantitatively the adsorption of crytl violet by bacteria. In addition, the effect of the species and strain of the organism used, the...