A novel gene encoding transglycosylating beta-galactosidase (BGase) was cloned from Penicillium expansum F3. The sequence contained a 3,036-bp open reading frame encoding a 1,011-amino-acid protein. This gene was subsequently expressed on the cell surface of Saccharomyces cerevisiae EBY-100 by galactose induction. The BGase-anchored yeast could directly utilize lactose to produce galactooligosa...

It is well known fact that CRP measurements may be helpful in grading patients into high risk and low risk category for predicting future cardiovascular and neurovascular events. The current research focused the evaluation and the predictive value of hs-CRP (high sensitivity C – Reactive Protein) in relation to the ultimate functional outcome in first ever ischemic stroke after 4 weeks and to c...

Graphene oxide (GOs) has emerged in recent years as a versatile nanomaterial, demonstrating tremendous potential for multifunctional biomedical applications. GOs can be prepared by the top-down or bottom-up approach, which leads to a great variability of GOs being produced due to the different procedures and starting carbon sources adopted. This will have an effect on the physiochemical propert...

This study aimed to investigate the effects of galacto-oligogalactose (GOS) glycosylation on structural and functional properties fish gelatin (FG). Results showed that with increase time, grafting degree browning increased, new protein bands increased molecular weight were observed by SDS-PAGE. Structural analysis reduced intrinsic fluorescence intensity surface hydrophobicity FG. FTIR α-helix...

Beta-galactooligosaccharides (GOS) are oligosaccharides normally produced industrially by transgalactosylation of lactose. They are also present naturally in the milk of many animals including humans and cows. GOS are thought to be good for health, being potential prebiotic fibres, and are increasingly added to food products. In order to control the GOS content of products, the AOAC official me...

Abstract: The objective of this article is to construct evidence-based arguments on what comprehension and are various instructional approaches improve post the National Reading Panel’s (NRP) report (2000). NRP (2000) reported seven text instructions that have scientific evidences in improving reading comprehension. Those are: (1) monitoring, (2) cooperative learning, (3) graphic semantic organ...

Valorization of by-products in the dairy industry as a strategy to promote circular economies has become priority around globe. Cheese whey and its derivates from filtration technologies offer source valuable molecules such proteins, fat, lactose, minerals. For this study, after protein concentration recovery through ultrafiltration, lactose remaining permeate was furtherly concentrated with na...

In this 30-day experiment, the effect of probiotics (Bacillus clausii, commercial probiotics: Enterogermina) and prebiotics (Galactooligosaccharides, GOS) on growth red cherry shrimp (Neocaridina davidi) has been investigated for 30 days. The trial was conducted with 270 shrimps (initial weight: 0.24±0.03 g) in 27 plastic tanks (volume 0.01 m3 area 0.05 m2) representing 9 groups. Except control...

Using germ-free rats inoculated with a human faecal flora (gnotobiotic rats), the effects of three oligosaccharides (beta-fructo-oligosaccharides (FOS), beta-galacto-oligosaccharides (TOS) and alpha-gluco-oligosaccharides (GOS)) on intestinal bacterial metabolism were compared. The animals were fed on either a control diet or diets containing 40 g/kg of GOS, FOS or TOS. FOS and TOS were the pre...