Chromosomal patterns of experimental animals are useful tools for cytogenetics research and animal breeding. Chromosome investigations of the hairless guinea pig are rare, therefore, karyotype of hairless guinea pigs (twelve male and female) was studied using metaphase spreads of bone marrows and G banding techniques. The chromosomes diploid number was 2n= 64 and polymorphism of three type chro...

Purified sarcolemmal and light vesicle (intracellular) fractions of/3-adrenergic receptors were used to examine the effects of propranolol on receptor translocation in guinea pig heart. Guinea pigs were given propranolol (0.15 mg/kg/hr) via minipumps for 7 days and either killed or made ischemic for 1 hour via a coronary ligature. Propranolol treatment led to an externalization of ̂ -receptors f...

Activity of the flavin-containing monooxygenase (FMO) was reduced significantly in ascorbic acid deficient guinea pigs. Reduction in oxidation of dimethylaniline (DMA) and of thiobenzamide was associated with a decrease in the activity of the FMO. In both ascorbate supplemented and deficient guinea pig hepatic 12,000 g supernatant fractions, SKF-525A and n-octylamine did not inhibit DMA N-oxida...

Purified sarcolemmal and light vesicle (intracellular) fractions of beta-adrenergic receptors were used to examine the effects of propranolol on receptor translocation in guinea pig heart. Guinea pigs were given propranolol (0.15 mg/kg/hr) via minipumps for 7 days and either killed or made ischemic for 1 hour via a coronary ligature. Propranolol treatment led to an externalization of beta-recep...

1. The method of Kekwick & Mackay (1954) for the fractionation of human plasma protems was used successfully for the separation of the serum proteins ofguinea pig, rat, rabbit, cat, dog, monkey, ox, horse and sheep. 2. By variations in pH, ionic strength, temperature and solvent concentration, three fractions consisting essentially of a mixture of xand #globulins (G2); y-globulin (G 3) and albu...

It was reported previously that the incubation of normal guinea pig macrophages with partially purified products of activated lymphocytes resulted in altered macrophage function including increased cell adherence to culture vessels, spreading, phagocytosis, and glucose carbon-1 oxidation. Studies reported here demonstrate that such macrophages also exhibit enhanced bacteriostasis. Lymphocytes w...

AIM OF THE STUDY This study was undertaken to provide a pharmacological basis for traditional use of Acorus calamus in airways disorders. MATERIALS AND METHODS Isolated guinea-pig trachea and atria were suspended in organ baths bubbled with carbogen and mechanisms were found using different parameters. RESULTS In isolated guinea-pig tracheal segments, crude extract of Acorus calamus was mor...

The usually accepted mothods for bioassay of histamine according to Schild el at. (1951), employ (i) eat's blood pressure, (ii) guinea pig's uterus or (iii) the guinea pig's ileum. Code (1952) has reported that the most convenient tissue for the bioassay of histamine is guinea pig's terminal portion of the ileum and on this account it is the most widely employed pharmacological preparation for ...

The leaf base of Sorghum bicolor (Family: Gramineae, Poaceae) was cold-macerated with 70% v/v methanol. The aqueous methanolic extract was further fractionated into non-polar, medium polar and very polar components using hexane, ethylacetate and water (aqueous), respectively. The gastrointestinal effects of these extracts were tested on intestinal motility (transit) in mice, castor oilinduced d...

Ribosomal fractions obtained from Mycobacterium bovis (BCG) and M. smegmatis (strain butyricum) were studied to determine their antigenicity, their ability to stimulate the production of soluble mediators of delayed hypersensitivity (in vitro correlates) by sensitized peritoneal exudate cells, and the antigenic relations of ribosomal antigens of BCG to BCG protoplasm and H37Rv culture filtrates...