Continuous circulation of influenza A(H5N1) virus among poultry in Egypt has created an epicenter in which the viruses evolve into newer subclades and continue to cause disease in humans. To detect influenza viruses in Egypt, since 2009 we have actively surveyed various regions and poultry production sectors. From August 2010 through January 2013, >11,000 swab samples were collected; 10% were p...

Sequence analysis and phylogenetic study of hemagglutinin (HA) gene of H9N2 subtype of avian influenza virus isolates (outbreaks of 1998-2002) in Tehran province (Iran) were studied. Two sets of forward and reverse primers in highly conserved regions, based on sequences of HA gene in Genbank, were designed. PCR products of a 430-bp fragment of 16 isolates were sequenced and then were aligned wi...

The aim of the present was to evaluate the prevalence of H9N2-specific antibodies among water buffaloes (Bubalus bubalis). To this end, blood samples were obtained from 80 randomly selected water buffaloes, 40 cases of which were obtained in the winter months, and 40 cases were sampled in the spring months. The presence of H9N2-specific antibody was determined by hemaggl...

background: the h9n2 subtype of avian influenzaviruses (aivs) have spread in asia and middle east countriesand have become a serious threat to poultry industry in iran.objectives:characterization of genes of h9n2 subtype involvingin pathogenicity and diagnosis are crucial in control of avianinfluenza outbreaks. the nonstructural (ns) gene and its proteinproducts (ns1 & ns2) are important as...

Phylogenetic analysis of influenza viruses collected during December 2009-February 2010 from chickens in live poultry retail shops in Lahore, Pakistan, showed influenza A(H9N2) lineage polymerase and nonstructural genes generate through inter- and intrasubtypic reassortments. Many amino acid signatures observed were characteristic of human isolates; hence, their circulation could enhance inter-...

An H9N2 virus lethal to chickens was isolated from an acutely ill chicken flock in 2012. Phylogenetic analyses indicated that this virus was phylogenetically related to the Y280 lineage. Sequence analysis showed 1 amino acid deletion in HA1 and 3 amino acid deletions in the NA stalk region.