In this work, biocompatible ionic liquids based on aminoacids were employed as extractants to separate extremolipases from aqueous streams. First, the influence of aminoacid and dipeptide-based (cholinium glycinate, ChGly, cholinium glycylglycinate, ChGlygly) lipolytic activity a commercial lipase Candida antarctica (CaLB) in-house synthesized extremophilic lipases Thermus thermophilus HB27 (Tt...

Abstract The aim of the study was to characterize halotolerant bacteria and evaluate their plant growth promotion potential on chia quinoa seedlings under saline stress. Isolated microorganisms were evaluated for nitrogen fixation, phosphate solubilization, production siderophores indole acetic acid. Three strains two consortia selected: Halomonas sp. (SFS), Micrococcus luteus (SA211), Bacillus...

We have established a transposon mutagenesis procedure for the moderate halophile Halomonas eurihalina, a bacteria that produces an exopolysaccharide (EPS) of considerable biotechnological interest. We used suicide plasmids pUT and pSUP102 to introduce the transposons mini-Tn5 and Tn1732 into H. eurihalina via Escherichia coli mediated conjugation. Southern hybridization analysis demonstrated t...

Among the 30 strains of moderately halophilic bacteria isolated from the salty effluents of textile industries around Qom city in central Iran, a Gram-negative rod shape bacterium designated as strain IP8 showed a remarkable ability in decolorizing of azo dyes over wide ranges of pH (7-11) and temperature (25-45 ºC), in presence of NaCl and Na2SO...

A high-throughput screening system for moderately halophilic phenol-degrading bacteria from various habitats was developed to replace the conventional strain screening owing to its high efficiency. Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes. Measurement of phenol concentrations was performed in 96-well microplates instead of using the conv...