Fluxes of ions and water across the short-circuited, excised bullfrog lung were determined by radioisotope techniques. The unidirectional flows of Na+, K+, Ca++, TcO4 minus, HCO3 minus, gluconate, rho-aminohippurate, dinitrophenolate, SO4 equal to, and water were symmetrical. Both HCO3 minus fluxes were reduced by acetazolamide. In contrast, Cl minus, Br minus I minus, and SCN minus movement fr...

The hydrogeochemical characteristics of groundwater are an important element in the study spatial and temporal variation resources, which is crucial to water resources utilization, ecological environmental protection, human development. Water samples were collected at eight observation Nandong Karst System (NKWS) sites each month 2019, main ions isotopes examined. hydrogeochemistry its differen...

Groundwater aquifers as one of the main sources of water supplies are faced with different risks such as level dropping due to lack of precipitation, and natural and non-natural pollutants. Thus, it is extremely necessary to monitor ground water quality. In the present study, the concentration of cations, anions, and some toxic metals was evaluated in 21 rural water supplies in Qorveh plain in ...

We investigated in a physiological salt solution (PSS) containing HCO3the intracellular pH (pHi) regulating mechanisms in smooth muscle cells cultured from human internal mammary arteries, using the pH-sensitive dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) and 22Na' influx rates. The recovery of pH; from an equivalent intracellular acidosis was more rapid when the cells were in...

Burmese pythons are sit-and-wait predators that are well adapted to go long periods without food, yet subsequently consume and digest single meals that can exceed their body weight. These large feeding events result in a dramatic alkaline tide that is compensated by a hypoventilatory response that normalizes plasma pH; however, little is known regarding how plasma HCO3(-) is lowered in the days...

Measurements of Pb, Cu, Zn, As, Ni, Hg, and Fe heavy metals and major elements and components such as Ca, HCO3, SO4, Na, K, Mg and Cl in soluble rain fractions were performed on rainwater collected at Arak plain during the rainy seasons of 2012. Concentrations of the heavy metals in the soluble fractions decreased for Fe, Pb, Zn, Ni, Cu, As and Hg, respectively and the major elements and compon...

Bestrophin-1 (Best1) is a Cl(-) channel that is linked to various retinopathies in both humans and dogs. Dysfunction of the Best1 Cl(-) channel has been proposed to cause retinopathy because of altered Cl(-) transport across the retinal pigment epithelium (RPE). In addition to Cl(-), many Cl(-) channels also transport HCO3(-). Because HCO3(-) is physiologically important in pH regulation and in...

One of the major tasks of the renal proximal tubule is to secrete acid into the tubule lumen, thereby reabsorbing approximately 80% of the filtered HCO3- as well as generating new HCO3- for regulating blood pH. This review summarizes the cellular and molecular events that underlie four major processes in HCO3- reabsorption. The first is CO2 entry across the apical membrane, which in large part ...

Na movement across the plasma membranes of confluent monolayers of monkey kidney epithelial cells (BSC-1) was studied using 22Na+ uptake and efflux techniques in the presence of 10(-4) M ouabain. In the presence of 28 mM bicarbonate, uptake was inhibited by both 10(-3) M amiloride and 10(-3) M 4,4'diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). In DIDS-pretreated cells, 10(-3) M amiloride l...

We have previously demonstrated that mouse proximal tubules in vitro respond to changes in luminal flow with proportional changes in Na+ absorption (Du Z, Duan Y, Yan Q, Weinstein AM, Weinbaum S, and Wang T. Proc Natl Acad Sci USA 101: 13068-13073, 2004). It was hypothesized that brush-border microvilli function as a sensor to detect and amplify luminal hydrodynamic forces and transmit them to ...