Treatment of sheep erythrocytes with suitable concentrations of tannic acid render them capable of adsorbing certain protein molecules from solution in saline. Red cells which have adsorbed proteins in this way are agglutinated after washing by the homologous antiprotein sera, even by high dilutions. Through hemagglutination sera can be titrated for antibodies against antigens adsorbed on the c...

This work presents the results of the detection of antibodies (immunoglobulin G) for subtypes I and VI of VEE viruses complex (Togaviridae family) in people from the General Belgrano island, Formosa province (Argentina). The prevalence of neutralizing (NT) antibodies for subtype VI was from 30% to 70% and the prevalence of antibodies inhibitory of hemagglutination (HI) was of 0% in the first an...

in the present study the distribution of rubella hemagglutination inhibition (hi) antibody titer in the sera of 1486 women of 18 to 25 years old residing in tehran were examined. sera were pretreated with acid washed kaolin and pigeon red blood cell. titers below 1:10 and in some studies below 1:20 are considered nonimmune to rubella. our results show that 10, 29% of studied population had hi a...

Recombinant soluble trimeric influenza A virus (IAV) hemagglutinin (sHA(3)) has proven an effective vaccine antigen against IAV. Here, we investigate to what extent the glycosylation status of the sHA(3) glycoprotein affects its immunogenicity. Different glycosylation forms of subtype H5 trimeric HA protein (sH5(3)) were produced by expression in insect cells and different mammalian cells in th...

Haemagglutination is a specific form of agglutination and is used when antibodies bind to red blood cells, which act as a particulate antigen. Red blood cells are particularly useful targets as they are readily available and agglutination is observable using the naked eye. This technique is commonly used to determine the titre of an antibody (Ab), for blood grouping and viral quantification. In...

OBJECTIVE To determine the intention to use hearing aids (HAs) by applying the theory of planned behavior (TPB). DESIGN The TPB is a widely used decision-making model based on three constructs hypothesized to influence the intention to perform a specific behavior; namely, "attitude toward the behavior", "subjective norm", and "behavioral control". The survey was based on a TPB-specific questi...

The temperature at which the coupling of antigen to erythrocytes takes place is an important factor in the passive hemagglutination test for hepatitis B antibody. Erythrocytes sensitized at 16 C are much less sensitive for the detection of antibody than are those sensitized at 22 to 41 C.

OBJECTIVES To assess cognition in populations born and living at high altitude (HA; 3,700 m) and low altitude (LA; 500 m) in Bolivia, who were similar for both socioeconomic status and genetic ancestry. To determine whether HA hypoxia influences cognitive decline across the life span. METHOD In total, 191 healthy participants aged 4 to 85 years were assessed at HA (N = 94; 33; 35% male) and L...

This radioimmunoassay was developed for specific and large-scale routine measurement of autoantibodies to thyroperoxidase (TPO), an enzyme recently identified as the thyroid microsomal antigen. Because of the scarcity of purified thyroperoxidase, we did not base the assay on the antigen-coated method but rather on autoantibody inhibition of the binding of labeled TPO to a solid-phase-bound mono...