Open Access Poster presentation Nitric oxide-independent vasodilator rescues heme-oxidized soluble guanylate cyclase from proteosomal degradation Sabine Meurer1,2, Sylke Pioch2, Tatjana Pabst2, Nils Opitz1,2,3, Peter M Schmidt1,4, Tobias Beckhaus5, Kristina Wagner2, Simone Matt2, Kristina Gegenbauer1,6, Sandra Geschka7,8, Michael Karas5, JohannesPeter Stasch7,9, Harald HHW Schmidt1 and Werner M...

Treatment of mouse hepatoma (Hepa) cells with heme or cadmium chloride in serum-free medium causes a rapid increase in the steady-state level of heme oxygenase (HO) messenger RNA. This increase is both dose- and time-dependent. Maximum accumulation of HO mRNA is observed 3 h after addition of either agent. Treatment of Hepa cells with heme or CdCl2 also stimulates the transcription of the HO ge...

OBJECTIVE Carbon monoxide (CO) is generated from vascular smooth muscle cells via the degradation of heme by the enzyme heme oxygenase-1. Since smooth muscle cell apoptosis is associated with numerous vascular disorders, we investigated whether CO regulates apoptosis in vascular smooth muscle. METHODS AND RESULTS Treatment of cultured rat aortic smooth muscle cells with a combination of cytok...

In the absence of external electron donors, oxidized bovine cytochrome c oxidase (CcO) exhibits the ability to decompose excess H2O2. Depending on the concentration of peroxide, two mechanisms of degradation were identified. At submillimolar peroxide concentrations, decomposition proceeds with virtually no production of superoxide and oxygen. In contrast, in the millimolar H2O2 concentration ra...