A significant elevation of cathepsin D activity was observed in six human hepatoma tissues as compared to 12 normal human livers. In isoelectric focusing experiments, cathepsin D purified from normal liver exhibited three different forms, with isoelectric points of 5.6, 6.1, and 6.7, while cathepsin D purified from hepatoma contained another five to six more acidic forms in addition to the form...

Hepatomas occurred in the liver of 5 of 386 ACT rats at the site of injection, 10—16weeks previously, of cells of regenerating liver pre-incubated with I)NA derived from the Morris hepatoma 3924C, transplantable in this strain. A tumor of undetermined nature developed in 1 of 62 rats 6 months after intrahepatic injection of a mixture of preneoplastic liver DNA and RNA. No tumors occurred in 1...

The activities of glucose-6-phosphatase, glutamate dehydrogcnase, and choline ox[dase were determined in some or all of ten rat hepatomas, including the Novikoff, Dunning L-C18, McCoy MDAB, and the Morris 3683, 39524A, and 51~3 hepatomas, together with primary hepatomas produced by feeding ethionine or 3%nethyl-4dimethylaminoazobenzene, and transplanted hepatomas derived from the primary tumors...

Growing evidence indicates that the deregulation of microRNAs (miRNAs) contributes to the tumorigenesis. We previously revealed that microRNA-520b (miR-520b) was involved in the complement attack and migration of breast cancer cells. In this report, we show that miR-520b is an important miRNA in the development of hepatocellular carcinoma (HCC). Our data showed that the expression levels of miR...

Occult hepatitis B virus (HBV) infection is defined as persistence of HBV DNA in liver tissues, with or without detectability of HBV DNA in the serum, in individuals with negative serum HBV surface antigen (HBsAg). Despite accumulating evidence suggesting its important clinical roles, the molecular and virological basis of occult hepatitis B remains unclear. In an attempt to establish new hepat...

Studies on carbohydrate metabolism have re vealed important differences between hepatoma and normal liver. It has been pointed out by Olson (14) that the carbohydrate metabolism of the hepatoma is al tered in such a way that glucose is diverted almost entirely from its storage as glycogen to its degra dation to lactic acid. Olson demonstrated increased phosphohexo-isomerase activity in the hepa...