identification and use of more efficient enzymes in the food and pharmaceutical industries is the focus of many researchers. the aim of this study was to search for a new bacterial strain capable of producing high levels of pullulanase applicable to biotechnology, the starch bioprocessing and food industries. a new pullulan hydrolyzing bacillus strain was isolated and designated sdk2. morpholog...

Twelve isolates of Pithomyces spp. from Texas were tested for sporidesmin toxin production, using both high-performance and thin-layer chromatography techniques. None of the Texas isolates produced the toxin under the conditions used. A control toxigenic New Zealand isolate, Pithomyces chartarum strain C, was grown simultaneously under the conditions tested and was found to produce sporidesmin ...

from hexanic extract of aerial parts of peucedanum ruthenicum m. bieb (apiaceae) collected from mazandaran province of iran, four triterpenoids namely taraxasterol, y-taraxasterol, poriferasterol and b-sitosterol have been isolated by column chromatography, thin layer chromatography, and crystallization. their structures were elucidated by spectroscopic methods (uv, ir, ms, 1h and 13c-nmr).

The measurement of glycosylated haemoglobin in blood is vital for monitoring metabolic control in diabetics [1-3]. The development of many different methods of measurement (based on cation-exchange chromatography, electrophoresis, high performance liquid chromatography [HPLC], affinity chromatography, radio and colorimetric immuno techniques) proves the importance of this assay [4]. Since the f...

In this present situation increase in the number of diseases has been observed and several new medications are invented have developed to treat various disorders, which approved by FDA. But before these drugs come market it must undergo procedures. The validation analytical process a drug development helps ensuring its purity reliability. This involves use techniques collect data about drug. re...

We have developed a simplified and improved high performance liquid chromatography (HPLC) method for the detection and quantitation of tissue lipid using a new laser light-scattering detector (Varex model ELSD II). This detector has a limit of sensitivity of 50 ng for neutral lipid and 200 ng for most phospholipids with excellent reproducibility. By coupling the ELSD II with a ternary gradient ...

the efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. conclusions were drawn regarding selection of solid phase according to pore size, rigidity, ph stability, chemistry of derivation and activation, and gross concentration of immobilized ligand required fo...

background: a simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. methods: the mobile phase composed of acetonitrile-water 60:40 (v/v) and 0.2% formic acid 20 µl of sample was chromatographically analyzed using a repacked zorbax-xdb-ods c18 column (2.1 mmx30...

background: the illicit vials with street name temgesic and norgesic has been distributed in black market of iran in recent years. with reference to their name, one may presume that they contain the opioid buprenorphine but there are some reports in opposition to this. true nature of these vials has been shown in the current study. methods: all of drugs that had been apprehended by law enforcem...