Citrate synthase (CS) plays a central metabolic role in aerobes and many other organisms. The CS reaction comprises two half-reactions: a Claisen aldol condensation of acetyl-CoA (AcCoA) and oxaloacetate (OAA) that forms citryl-CoA (CitCoA), and CitCoA hydrolysis. Protein conformational changes that `close' the active site play an important role in the assembly of a catalytically competent cond...

Knowledge of the response of cytochrome P450 1B1 (CYP1B1) to exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in both humans and rodents is limited. To improve the analysis of CYP1 proteins, specific CYP1B1 and CYP1A1 polypeptides were expressed as hexahistidine-tagged fusion proteins in Escherichia coli, purified to homogeneity and used to produce polyclonal antibodies in rabbits. Immuno...

Tandem affinity strategies reach exceptional protein purification grades and have considerably improved the outcome of mass spectrometry-based proteomic experiments. However, current tandem affinity tags are incompatible with two-step purification under fully denaturing conditions. Such stringent purification conditions are desirable for mass spectrometric analyses of protein modifications as t...

kashf al-mahjoub is the first persian independent mystical text, written by abu al-hasan ali bin osman al-jolabi al-hojwiri al-qaznavi which contains valuable information about different subjects of sufism. since one of the effective methods on which one can understand a sufi’s viewpoints and can figure out his ideology and even his anagogic and mystical psychology is to analyze his esoteric in...

In this keynote lecture the author presents a research ideology, a novel multi-paradigm methodology, and advanced computational models for automated electroencephalogram (EEG)-based diagnosis of neurological disorders. The methodology is based on adroit integration of three different computing technologies and problem solving paradigms: neural networks, wavelets, and the chaos theory. Examples ...

In his book, Analytical Archaeology, Clarke (1968:512-513) noted three ways in which mathematical concepts are relevant to what he calls archaeological ideology: "(i) The need for entitation and quantification ... using ... descriptive statistics"; "(ii) The need to handle relationship concepts ... using analytical inductive statistics"; "(iii) The need to handle the regularities in complex dat...

The present work seeks, through the publications of research by authors from different areas knowledge, such as area ​​Arts and Human Sciences, to bring elucidations debates on relationship between arts, in particular music Nazi regime, beginning. party until collapse Germany at end war. Through bibliographic review, we try notes here relations music, Wagner’s opera, with passing German people ...

Does popular ideology affect public policies enacted in a country? The baseline argument links popular ideology input to policy output. Either a population more often elects a party with its favored ideology into office, which then implements policies in line with popular ideology. Alternatively, all parties pursue policies more in line with popular ideology. Under the first view, popular ideol...