Monoaminergic innervation of the intermediolateral nucleus of the cat spinal cord was investigated by fluorescence histochemistry and electron microscopy. Large numbers of monoaminergic terminals were labeled by prior administration of the false neurotransmitter 5-hydroxydopamine (5-OHDA). Ultrastructurally, 5-OHDA-labeled terminals fell into three types. Type I, which made up 55% of the labele...

The purpose of this study was to analyze the neuronal density of the myenteric plexus of the intermediate and antimesocolic regions of the descending colon of rats. Whole-mounts were stained with three different techniques of neuronal evidenciation. Through counts of the number of neurons in an area of 6.64 mm under light microscopy, we found 1,271 +/- 227.54 neurons with Giemsa in the intermed...

Chronic and non-healing skin wounds represent a significant clinical, economic and social problem worldwide. Currently, there are few effective treatments. Lack of well-defined animal models to investigate wound healing mechanisms and furthermore to identify new and more effective therapeutic agents still remains a major challenge. Pig skin wound healing is close to humans. However, standardize...

This report concerns the observation that certain anionic detergents have a specific affinity for elastica, and because these anionic detergents precipitate cationic basic dyes in aqueous solution, a rather specific stain for elastica can be obtained. There are many satisfactory methods for the histologic demonstration of elastica, the most popular being: a) acid orcein (Unna), b) Verhoef's, c)...

Phosducin (Pd), a small protein found abundantly in photoreceptors, is widely assumed to regulate light sensitivity in the rod outer segment through interaction with the heterotrimeric G protein transducin. But, based on histochemistry and Western blot analysis, Pd is found almost entirely in the inner segment in both light and dark, most abundantly near the rod synapse. We report a second smal...

Since Steedman ( 1950) has introduced it into histochemistry, alcian blue has been advocated to reveal a staining selectivity which depends upon the presence of acidic groups in mucopolysaccharides (Mowry, 1956; Pearse, 1960). As a plausible ground of this concept the fact has been presented that the alcian blue staining of polysaccharides can be increased in intensity, when acidic groups are i...

Because of their diverse habitats, crabs are excellent experimental species to study owing to the morphological changes and physiological adaptation that occur during their terrestrial invasion. Their hemolymphic osmoregulation in brackish water is crucial for a successful terrestrial invasion. Crabs can actively uptake or excrete ions upon salinity change, and the gills play a major role among...

An unusual case of a mucin secreting benign microfollicular adenoma of the thyroid in a 30 year old euthyroid woman is reported. Histologically, the lesion was characterised by follicular cells with the appearance of signet ring cells. Histochemistry showed the mucin content of these cells to consist uniformly of sulphated acid mucins; positive thyroglobulin immunostaining was also shown. The p...

To identify cellular interactions that underlie the spatially appropriate transcription of neural genes, we characterized the embryonic development of proopiomelanocortin (POMC) gene expression in Xenopus laevis using in situ hybridization histochemistry. This has led to the establishment of a unique model system for studying how a neuropeptide gene program in four distinct cell groups is set u...

The bronchial glands in the main bronchus and succeeding generations of the inferior lingular airway have ben studied in 10 left lungs removed surgically for lung cancer. The ratios of sulphated to sialidated mucin found did not coincide with previous results and suggested that this is not a useful measurement for the assessment of cigarette smoke or other inhaled pollutants. The total acid muc...