The adult Wistar strain albino rats were vasectomised by conventional method and maintained for six months. The vasectomized rat testis had elevated water content with depleted dry matter. Glycogen content was increased with indication of mobilization of hexoses into HMP pathway. The vasectomized rat testis showed preferential utilisation of triglycerides. In view of increased 3 beta-HSD and 17...

We examined 5alpha-dihydrotestosterone (5alpha-DHT) inactivation and the expression of several steroid-converting enzymes with a focus on aldoketoreductases 1C (AKR1C), especially AKR1C2, in abdominal adipose tissue in men. AKR1C2 is mainly involved in the conversion of the potent androgen 5alpha-DHT to its inactive forms 5alpha-androstane-3alpha/beta,17beta-diol (3alpha/beta-diol). Subcutaneou...

Dehydrogenase (cortisol to cortisone) and oxoreductase (cortisone to cortisol) activities of porcine placental 11beta-hydroxysteroid dehydrogenase (11beta-HSD) were measured in tissue fragment cultures on day 75 of gestation. Dehydrogenase activity was over fivefold greater than oxoreductase activity (p < .001). There were positive linear associations (p < .01) between net dehydrogenase activit...

We found that 3α-hydroxysteroid dehydrogenase (3α-HSD) from Pseudomonas sp. B-0831 can catalyze the carbonyl reduction of non-steroid substrates, such as metyrapone and p-nitrobenzaldehyde, in addition to the activity of 3α-hydroxysteroid dehydrogenase. The results of an inhibition study in metyrapone reduction by androsterone and cholic acid indicated that metyrapone bound to the same catalyti...

A t a symposium titled “Adipose Tissue as a Secretory Organ” at the American Diabetes Association (ADA), San Francisco, June 2002, Jeffrey Flier (Boston, MA) discussed the effects of visceral obesity. While reviewing the differing components of the metabolic syndrome, which to large extent are driven by insulin resistance, he asked, “Where does the insulin resistance come from?” The existence o...

The localizations of steroidogenic enzymes (P450scc, 3 beta HSD, P450c17 and P450arom) in testes of Shiba goats were investigated by immunohistochemistry. P450scc, 3 beta HSD, P450c17 and P450arom were detected in all Leydig cells of adults. P450scc and P450c17 were observed in most Leydig cells in the fetus (90 days) and neonate (15 days). 3 beta HSD and P450arom were found in some Leydig cell...

The purpose of the study was to examine the effect of luteal macrophage conditioned medium (LMCM) on progesterone and estradiol production by cultured granulosa cells. Porcine granulosa cells were cultured for 48 h with or without LMCM in the absence or presence of 100 ng/ml LH, FSH or prolactin. Progesterone and estradiol concentrations were measured by radioimmunoassay. Granulosa cells were a...

A, androstenedione Arom, aromatase AMH, anti Müllerian hormone cAMP, adenosine 3': 5'cyclic monophosphate DHT, dihydrotestosterone E, estrogen E1, estrone E2, estradiol 17B ER, estrogen receptor FT, female-producing temperature 3B-HSD, 3B-hydroxysteroid dehydrogenase-5-ene-4-ene isomerase 17B-HSD, 17B-hydroxysteroid dehydrogenase hsp, heat-shock protein MT, male-producing temperature P 450 arom...

Dietary supplementation with royal jelly (RJ) may improve growth, antioxidant gene expression, and intestinal capacity, possibly mitigate effects of stress induced by high stocking density (HSD) in zebrafish (Danio rerio). Effects HSD RJ on growth performance, capacity were investigated. A total 240 four-month-old used a 2 x factorial design, four treatments three replications. The consisted no...

This study evaluates the energy efficiency of pressure shock damping in a hydraulic lifting and leveling (HLL) module mobile robotic bricklaying system (RBS). The HLL includes servohydraulic actuator (SHA) damper (HSD). proposed adjustable HSD consists accumulator circuit (HAC) proportional valve. frequency characteristics impedance indices were used to evaluate effectiveness damping. dynamic r...