Cerium oxide nanocrystals (CeO2-NCs) exhibit superoxide dismutase and catalase mimetic activities. Based on these catalytic activities, CeO2-NCs have been suggested to have the potential to treat various diseases. The crystalline size of these materials is an important factor that influences the performance of CeO2-NCs. Previous reports have shown that several metal-based nanocrystals, includin...

objective: this study aimed to evaluate the incidence of apoptosis in vitrified and non-vitrified human ovarian tissue by the use of morphological analysis and apoptosis assay techniques. methods: we obtained human ovarian tissue biopsies from 30 women who underwent elective caesarean sections. tissues were transported to the laboratory in pre-warmed, equilibrated leibovitz l-15 medium within 2...

PURPOSE To determine the effects of activated monocytes on the induction of human retinal pigment epithelial (HRPE) cell reactive oxygen metabolite (ROM) production and apoptosis. METHODS HRPE cells were co-cultured with interferon-gamma (IFN-gamma)-stimulated human monocytes. HRPE apoptosis was detected by propidium iodide, proliferating cell nuclear antigen (PCNA) and TdT-mediated dUTP nick...

The anticancer drug cyclophosphamide induces granulosa cell apoptosis and is detoxified by glutathione (GSH) conjugation. We previously showed that both cyclophosphamide treatment and GSH depletion induced granulosa cell apoptosis in rats, but the role of GSH in apoptosis in human ovarian cells has not been studied. Using the COV434 human granulosa cell line, we tested the hypotheses that (1) G...

Hypoxia contributes to cytotoxic chemotherapy and radiation resistance and may play a role in the efficacy of antiangiogenesis cancer therapy. We have generated a series of cell lines derived from the colon adenocarcinoma models HT29 and HCT116 by exposing cells in vitro to repeated sublethal periods of profound hypoxia. These cell lines have altered sensitivity to hypoxia-induced apoptosis: th...

Aims Studies have shown that probiotic bacteria inhibit the onset and progression of carcinogenesis through different pathways. Our objective in this study was to determine effect on expression growth-related genes Rel A, IKB, Casp3 HT29 colon cancer cells Methods & Materials In study, Lactobacillus brevis were first cultured, after supply media conditioning, they treated cells. The bacteri...

background: prostate cancer is one of the most commonly diagnosed cancers in males. tumor suppressor gene p53 plays an important role in causing cell cycle arrest and allowing apoptosis to proceed. objective: to investigate the expression of p53 protein and its relation to apoptosis and prostate cancer traditional prognostic indicators. methods: in this study expression of p53 was examined in p...

In vivo, butyrate is a major energy source for the colonic epithelium and is thought to stimulate proliferation. In contrast, butyrate in vitro has been shown to inhibit proliferation and induce differentiation and apoptosis in colonic epithelial cells. Most colon cell cultures are grown in medium containing high concentrations of glucose, whereas in vivo, the main energy source used by the col...

objective: cryopreservation of immature testicular tissue should be considered as an important factor for fertility preservation in young boys with cancer. the objective of this study is to investigate whether immature testicular tissue of mice can be successfully cryopreserved using a simple vitrification procedure to maintain testicular cell viability, proliferation, and differentiation capac...

Background and Purpose—Mutations in the Programmed Cell Death 10 (PDCD10) gene cause autosomal dominant familial cerebral cavernous malformations (CCM3). To date, little is known about the function of this gene and its role in disease pathogenesis. Methods—We examined the effects of overexpression of wild-type and 2 human disease-causing variants of PDCD10 on cell death using 3 different method...