The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SSGCID Protein Production Group at the University of Washington (UW-PPG) has developed a high-throu...

Helicobacter pylori is motile by means of polar flagella, and this motility has been shown to play a critical role in pathogenicity. The major structural flagellin proteins have been shown to be glycosylated with the nonulosonate sugar, pseudaminic acid (Pse). This glycan is unique to microorganisms, and the process of flagellin glycosylation is required for H. pylori flagellar assembly and con...

β-keto esters are used as precursors for the synthesis of β-amino acids, which are building blocks for some classes of pharmaceuticals. Here we describe the comparison of screening procedures for hydrolases to be used for the hydrolysis of β-keto esters, the first step in the preparation of β-amino acids. Two of the tested high throughput screening (HTS) assays depend on coupled enzymatic react...

A method for solving the scheduling problem for a class of cyclic systems with respect to throughput maximization is presented. A strictly cyclic mode of operation is considered, where the time offset between the start of consecutive jobs is always constant. All jobs have to follow an identical time scheme. The time scheme may be restricted by due dates or time window constraints. There are no ...

Polymer micelles have been used widely in the delivery of various therapeutic drugs, which are also known as active pharmaceutical ingredients (APIs). Recent advances in drug discovery technology, including an accumulated database on therapeutic targets, combinational chemistry, and high-throughput screening (HTS), have further increased the number of candidate compounds and constructed a huge ...

Generating chemical libraries in droplet form is vital to the adoption of microdroplet systems in high-throughput screening (HTS). This paper presents microfractionation in droplets (μFD), a method for generating heterogeneous droplet libraries using standard separation processes. In μFD, compounds eluting from a separation column are permanently encapsulated into droplets by a droplet generato...

Diversity, in the form of genetic diversity, chemical diversity etc, is a very important concept in several areas of scientific research, and calculation of diversity is one of the most important considerations in pre-clinical drug discovery research and, in particular, in design of diverse chemical libraries for combinatorial chemistry and compound selection for High Throughput Screening (HTS)...

In cytomics bookkeeping of the data generated during lab experiments is crucial. The current approach in cytomics is to conduct High-Throughput Screening (HTS) experiments so that cells can be tested under many different experimental conditions. Given the large amount of different conditions and the readout of the conditions through images, it is clear that the HTS approach requires a proper da...

It is known that the ATP-binding cassette transporter A1 (ABCA1) plays a major role in cholesterol homeostasis and high density lipoprotein (HDL) metabolism. Several laboratories have demonstrated that ABCA1 binding to lipid-poor apolipoprotein A-I (apoA-I) will mediate the assembly of nascent HDL and cellular cholesterol efflux, which suggests a possible receptor-ligand interaction between ABC...