The lymphatic vessel endothelial receptor LYVE-1 is implicated in the uptake of hyaluronan (HA) and trafficking of leukocytes to draining lymph nodes. Yet LYVE-1 has only weak affinity for hyaluronan and depends on receptor clustering and higher order ligand organization for durable binding in lymphatic endothelium. An unusual feature of LYVE-1 not found in other HA receptors is the potential t...

We recently purified the rat liver hyaluronan receptor for endocytosis (HARE) and found abundant expression of 175- and approximately 300-kDa HARE species in sinusoidal endothelial cells of the liver, spleen, and lymph nodes. We report herein the first cloning and functional expression of the rat 175-kDa HARE. Peptide sequences were obtained from the purified 175-kDa HARE, and degenerate oligon...

Upon tissue injury, high m.w. hyaluronan (HA), a ubiquitously distributed extracellular matrix component, is broken down into lower m.w. (LMW) fragments, which in turn activate an innate immune response. In doing so, LMW HA acts as an endogenous danger signal alerting the immune system of a breach in tissue integrity. In this report, we demonstrate that LMW HA activates the innate immune respon...

Purpose The aim of the present prospective study was to investigate the efficacy of hyaluronan (HA) using two different concentrations as embryo transfer media for in vitro fertilization embryo transfer cycles. Methods A total of 169 cycles undergoing fresh embryo transfer on day 2 or 3, 561 cycles undergoing frozen-thawed embryo transfer on day 2 or 3, and 484 cycles of frozen-thawed blastoc...

An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism, thus providing a flexible imaging strategy for monitoring disease and repair...

BACKGROUND Lung cancer is a devastating disease with limited treatment options. Many lung cancers have changes in their microenvironment including upregulation of the extracellular matrix glycosaminoglycan, hyaluronan (HA), which we have previously demonstrated can regulate the activity of the extracellular serine protease, hyaluronan binding protein 2 (HABP2). This study examined the functiona...

Idiopathic pulmonary arterial hypertension (IPAH) is a fatal disease characterised by elevated blood pressure in the pulmonary circulation. Initial vasoconstriction, proliferation of pulmonary arterial smooth muscle cells (PASMC) and increased deposition of extracellular matrix (ECM) contribute to pathological remodelling of pulmonary arterioles in IPAH. Glycosaminoglycans (GAGs), components of...

Objective: HYBID (Hyaluronan Binding Protein Involved in HA Depolymerization, KIAA1199) is a hyaluronan (HA) binding protein, which involves in depolymerization of HA. It is reported that HYBID mRNA is expressed in the lung, heart, skin and brain in murine and human. However, the role of HYBID in the brain remains unclear. In this study, we have made HYBID KO mice and evaluated its function in ...

Objective: HYBID (Hyaluronan Binding Protein Involved in HA Depolymerization, KIAA1199) is a hyaluronan (HA) binding protein, which involves in depolymerization of HA. It is reported that HYBID mRNA is expressed in the lung, heart, skin and brain in murine and human. However, the role of HYBID in the brain remains unclear. In this study, we have made HYBID KO mice and evaluated its function in ...