Plant cell walls are complex structures composed of high-molecular-weight polysaccharides, proteins, and lignins. Among the wall polysaccharides, cellulose, a hydrogen-bonded β-1,4-linked glucan microfibril, is the main load-bearing wall component and a key precursor for industrial applications. Cellulose is synthesized by large multi-meric cellulose synthase (CesA) complexes, tracking along co...

Because of its high biocompatibility, bio-degradability, low-cost and easy availability, cellulose finds application in disparate areas of research. Here we focus our attention on the most recent and attractive potential applications of cellulose in the biomedical field. We first describe the chemical/structural composition of cellulose fibers, the cellulose sources/features and cellulose chemi...

The crystallinity of cellulose is one of the main structural parameters determining properties like accessibility and reactivity of cellulose. Native cellulose (e.g., wood, pulp, cotton, kenaf, bacteria cellulose) consists of the cellulose I, regenerated cellulose consists of the cellulose II modification. WAXS (wide angle X-ray scattering) is the standard method for the determination of crysta...

Cellulases are enzymes that normally digest cellulose; however, some are known to play essential roles in cellulose biosynthesis. Although some endogenous cellulases of plants and cellulose-producing bacteria are reportedly involved in cellulose production, their functions in cellulose production are unknown. In this study, we demonstrated that disruption of the cellulase (carboxymethylcellulas...

Novel thin and smooth deuterated cellulose films were synthesised to visualize adsorbed bio-macromolecules using contrast variation neutron reflectivity (NR) measurements. Incorporation of varying degrees of deuteration into cellulose was achieved by growing Gluconacetobacter xylinus in deuterated glycerol as carbon source dissolved in growth media containing D2O. The derivative of deuterated c...

background and objectives: research on xylanase has markedly increased due to its potential applications in pulping and bleaching processes using cellulose free preparations, textile processes, the enzymatic saccharification of lignocellulosic materials and waste treatment. the present study was aimed at isolation and characterization of xylan degrading strain of bacillus cereus from soil for p...

Although described some time ago, gamma-butyrobetaine esters and related compounds have not gained much attention from researchers, and their physiological function remains obscure. Formerly we detected GBB-esterase enzymatic activity in rat blood serum using phenylated gamma-butyrobetaine as an artificial substrate of the enzyme and HPLC. The aim of the present work was to develop an assay tha...

A study involving markers of subcellular and submitochondrial fractions, gradient centrifugation, latency measurements and extraction with digitonin, demonstrates the association of a specific ADP-ribose pyrophosphatase with rat liver mitochondria and its localization in the matrix space. The enzyme hydrolyses ADP-ribose to AMP, with a Km of 2-3 microM. The results support the occurrence of a s...

We report the pH-kobs profiles for the hydrolyses of phosphodichloridate and thiophosphodichloridate ions in aqueous solutions. Both species show broad pH-kobs plateaus that extend to high pHs. As part of an ongoing program to develop aqueous aminophosphorylation procedures, 1-3 we have investigated the reactivities of phosphodichloridate 1 and thiophosphodichloridate 2 ions as functions of pH ...

Comparative study was carried out on the production of protease using isolate from moraine. The isolates were screened on gelatin plates for gelatin hydrolyses by the different isolates. Among the all tested isolate No. 7 EHN produced the highest activity. while under the fermentation conditions after 3 days the maximum yield of protease was 277 U/ml. The protease production from marine isolate...