γ-Hydroxybutyric acid (GHB) is a recreational drug, a clinically prescribed drug in narcolepsy and alcohol dependence, and an endogenous substance that binds to both high- and low-affinity sites in the brain. For studying the molecular mechanisms and the biologic role of the GHB high-affinity binding sites, ligands with high and specific affinity are essential. The conformationally restricted G...

The in vitro cytotoxicity, stability, and metabolism of the 8-(N,N-dimethylcarboxamide) and 8-(N-methylcarboxamide) analogues of the experimental antitumor drug mitozolomide have been investigated in conjunction with their in vivo murine pharmacokinetics and metabolism. When tested against the TLX5 lymphoma in vitro the ID50 values for dimethylmitozolomide, methylmitozolomide, and mitozolomide ...

PURPOSE To investigate the intra-cameral level of ganciclovir following topical application of ganciclovir gel, 0.15% for cytomegalovirus (CMV) anterior segment infection. DESIGN Non-randomized, prospective, interventional clinical study. METHODS Patients with active CMV anterior segment infection seen at Singapore National Eye Centre, confirmed by positive CMV real time PCR (RT-PCR) of the...

BIGALKE, R. D ., SCHOEMAN, J. H . & McCULLY, R. M ., 1974. Immunization against bovine besnoitiosis with a live vaccine prepared from a blue wildebeest strain of Besnoitia besnoiti grown in cell cultures. 1. Studies on rabbits. Onderstepoort J. vet. Res. 41, 16 (1974). The immunizing potency of a live vaccine prepared from a strain of Besnoitia besnoiti isolated from blue wildebeest and stored ...

We investigated the kinetics of pulmonary angiotensin converting enzyme (ACE) in vivo by measuring the hydrolysis of a bolus dose of a synthetic ACE substrate, benzoyl-phenylalanyl-alanyl-proline (BPAP). Estimates of apparent Km and Vmax were calculated by multiple regression analysis using a nonlinear model of enzyme kinetics. A bolus injection of indocyanine green and BPAP was given into the ...

The γ-proteobacterium Francisella tularensis causes seasonal tick-transmitted tularemia outbreaks in natural rabbit hosts and incidental infections in humans in the south-central United States. Although Dermacentor variabilis is considered a primary vector for F. tularensis, Amblyomma americanum is the most abundant tick species in this endemic region. A systematic study of F. tularensis coloni...

abstract in order to study the sensitivity of seven crops to total herbicide soil residue, an experiment was carried out under controlled conditions in 2009 at the college of agriculture, ferdowsi university of mashhad-iran. in this study 7 crops (pea, bean, lentil, corn, rape, beet and tomato), across 7 simulated soil residual concentration of total herbicide (0, 0.0002, 0.0005, 0.001, 0.002, ...

Glycerophosphoinositol (GroPIns) is a major inositol phosphate in many cell types. In this study we have determined the optimal conditions (pH 8.0 and 0.5 mM MnCl2) for the metabolism of this molecule in an extract from human placenta, and we show that the major product is inositol (1)-phosphate (Ins(1)P). The enzyme activity that catalyzes this reaction is contained in the same protein designa...

IL-6-PE4E is a recombinant protein consisting of interleukin-6 (IL-6) fused to a mutant form of Pseudomonas exotoxin in which four basic amino acids are changed to glutamate (PE4E). The chimeric toxin has been previously shown to specifically kill malignant hepatic, prostatic, epidermoid, and myeloma cell lines in vitro. To explore the possible clinical utility of IL-6-PE4E, particularly as an ...

Prolonged fetal exposure to opioids results in neonatal abstinence syndrome (NAS), a major medical problem requiring intensive care and increased hospitalization times for newborns with NAS. Multiple strategies are currently available to alleviate withdrawal in infants with NAS. To prevent NAS caused by opioid maintenance programs in pregnant women, blocking fetal dependence without compromisin...