Hepatitis E virus (HEV) is one of the major causative agents of acute hepatitis in many developing countries. Recent intensive examination has revealed the existence of non-imported cases in industrialized countries. The patient was a 25-year-old Japanese female with acute hepatitis. Laboratory test demonstrated positive anti-nuclear antibody (ANA), anti-smooth muscle antibody (ASMA) and high l...

MUC1 is expressed at the cell surface of epithelial cancers. We have shown previously that MUC1 conjugated to keyhole limpet hemocyanin (KLH) plus the saponin immunological adjuvant QS-21 induces consistent high titer IgM and IgG antibodies in patients after treatment of their primary or metastatic cancers. KLH however is poorly soluble and heterogeneous making it difficult to work with, and we...

22 homosexual or narcotic addict patients at risk for acquired immunodeficiency syndrome (AIDS) or with AIDS, were studied for the presence of antiimmunoglobulin antibodies and circulating immune complexes (20 were thrombocytopenic, 6 had AIDS). Circulating immune complex levels were 10-fold higher than levels in normal subjects. IgG anti-F(ab')2 antibodies were noted in homosexual as well as n...

In order to help identify and characterize antigens involved in sperm functions and immune infertility, monoclonal antibodies (mAbs) were raised against human sperm surface antigens. The IgG fraction of serum from a donor with a spontaneous high titer of IgG-positive antisperm reactivity (as determined by immunobead binding) was purified by ammonium sulfate precipitation. This IgG preparation w...

BACKGROUND Brucellosis is a major public health problem in developing countries and has remained endemic in Iran. The aim of this study was to investigate the Seroprevalence of Brucellosis among high risk individuals in Guilan, Iran. METHODS In a cross-sectional study, 478 blood samples from people living in rural areas (n = 292) and 186 slaughterhouse workers were screened by slide agglutina...

The use of human sera collected from individuals of known infected and noninfected status is necessary for the validation of diagnostic assays and for the determination of cutoff values. However, the routine inclusion of pooled human sera from infected individuals for use as positive controls in commercial assay kits has many disadvantages. Sufficient quantities of sera can be difficult to obta...

Background: Scleroderma is a chronic systemic disorder that affects the connective tissues. It is characterized by several immune manifestations, inflammation, vascular damage, and fibrosis. Some of the viral infections with complex mechanisms are involved in the development and progression of many autoimmune diseases, such as scleroderma. The present study aimed to investigate the serological ...

Antibodies are essential tools of biomedical and biochemical researches. Polyclonal antibodies are produced against different epitopes of antigens. Purified F(ab')2 can be used for animal’s immunization to produce polyclonal antibodies. Human immunoglobulin G (IgG) was purified by ion exchange chromatography method. In all stages verification method of the purified antibodies was sod...

in endemic area the most challenging problem for brucellosis is to find a reliable diagnostic method. in this case-control study, we investigated the accuracy of elisa test for diagnosis of human brucellosis and determined the optimal cut-off value for elisa results in iran. the laboratory diagnosis of brucellosis was performed by blood isolation of brucella organism with a bactec 9240 system a...

Antibodies are a diverse class of glycoproteins that specially bind with antigen and elicit a number of secondary responses in vivo. Most of the applications of antibodies in basic research diagnosis and immunotherapy rely on labeling of the antibody with radiopharmaceuticals. Radiolabeled antibodies are a new class of imaging agents for the detection of sites of disease. True biodistribu...