Proteinpolysaccharide complex from bovine nasal cartilage exhibits a bimodal distribution of sedimentation coefficients when analyzed in the ultracentrifuge in associative solvents such as 0.5 M guanidinium chloride. The faster sedimenting mode disaggregates in dissociative solvents such as 4 M guanidinium chloride, giving a unimodal centrifugal pattern. Proteinpolysaccharide complex is separat...

Anti-Br was first identified in four cases of neonatal alloimmune thrombocytopenia (NAIT). The antigen Br is localized on the glycoprotein la/Ila complex of platelets. Anti-Br’ can best be detected by a glycoprotein-specific immunoassay using monoclonal antibodies for antigen immobilization (MAIPA assay) and radioimmunoprecipitation (RIP). Recently, we have identified sera from two polytransfus...

We have previously demonstrated the isolation of platelet membrane glycoprotein lIb-Illa by affinity chromatography with a specific monoclonal antibody.1 We have now separated the polypeptide subunits lIb and lIla of the isolated glycoprotein by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and have compared their structural features. Both llb and lIla contain -.1 5% car...

Anti-Br was first identified in four cases of neonatal alloimmune thrombocytopenia (NAIT). The antigen Br is localized on the glycoprotein la/Ila complex of platelets. Anti-Br’ can best be detected by a glycoprotein-specific immunoassay using monoclonal antibodies for antigen immobilization (MAIPA assay) and radioimmunoprecipitation (RIP). Recently, we have identified sera from two polytransfus...

Anti-Br was first identified in four cases of neonatal alloimmune thrombocytopenia (NAIT). The antigen Br is localized on the glycoprotein la/Ila complex of platelets. Anti-Br’ can best be detected by a glycoprotein-specific immunoassay using monoclonal antibodies for antigen immobilization (MAIPA assay) and radioimmunoprecipitation (RIP). Recently, we have identified sera from two polytransfus...

We thank all the participants for their help. Contributors: PHW and DGC had the idea for the paper and designed the study with support from KO and SJCT. JAG carried out the analyses. PHW drafted the paper, with contributions from all authors. PHW and DGC will act as guarantors for the paper. Funding: This study was funded by the Wellcome Trust (grant 051187/Z/97/A). Competing interests: None de...

Mutations in fukutin-related protein (FKRP) give rise to mild and more severe forms of muscular dystrophy. FKRP patients have reduced glycosylation of the extracellular protein dystroglycan, and FKRP itself shows sequence similarity to glycosyltransferases, implicating FKRP in the processing of dystroglycan. However, FKRP localization is controversial, and no FKRP complexes are known, so any FK...