To determine sensitivity and specificity of different antibodies for the immunocytochemical detection of malignant cells in diagnostically equivocal effusions in comparison with those achieved by DNA-image cytometry. 65 cytologically doubtful effusions of the serous cavities were stained with twelve antibodies. Furthermore, DNA-image cytometry was performed. Data were correlated with patient fo...

BACKGROUND Cytologic evaluation of the biliary tract strictures is nowadays widely used for distinguishing between benign and malignant lesions but remains a challenge for some problematic cases. Digital Image cytometry (DNA-cytometry) helps cytopathologists to resolve some unclear situations. METHODS We have analysed 41 specimens of bile duct brushings obtained from patients during ERCP (11 ...

DNA image cytometry was performed on Feulgen stained sections from 91 biopsies obtained during prospective endoscopic surveillance of 55 patients with Barrett's oesophagus. Aneuploid cells were detected in specimens from six of these patients. Four subsequently developed dysplasia and adenocarcinoma but, in the other two, biopsies had been reported as showing specialised epithelium only, with n...

The structure of the maize (Zea mays L.) root cap was studied to quantitatively evaluate the relationship among the size of the cells, their endopolyploidy level, and the abundance of the sucrose synthase isozyme SUS1. Median longitudinal root cap sections were analysed using immunolocalization, quantitative DNA staining, and image cytometry. Both the immunolocalization signal for the SUS1 prot...

This Application Note describes an alternative process for bacterial cell counting using on-chip fl ow cytometry with the Agilent 2100 Bioanalyzer system. This procedure is described with three different kinds of prokaryotes: Escherichia coli (gram-negative, rod-shaped), Pseudomonas aeruginosa (gram-negative, rod-shaped), and Staphylococcus aureus (gram-positive, coccal). As a reference for bac...

S RELATED TO THE SUBJECT OF THE THESIS Varga VS, Ficsor L, Galamb B, Kamarás V, Molnár B, Tulassay Z. Speed improvement of automated fluorescent digital slide scanning. 2008., 9th European Congress on Telepathology / 3rd International Congress on Virtual Microscopy, Toledo, Spain Varga VS, Ficsor L, Kamarás V, Monár B, Tulassay Z. Automated fluorescent slide scanning. 2007., 21th European Congr...

Methods. Forty-four surgically excised melanocytic lesions of the iris were histologically classified as nevus or melanoma. Morphometric analysis using a digital filar micrometer (LaSICO 1602N-10 and 5-4A) measured the mean size of the 10 largest nucleoli, and Feulgen staining and image cytometry (CAS 200 Cell Analysis Systems) analyzed the nuclear DNA ploidy in the lesions. Patient follow-up i...

Methods. Forty-four surgically excised melanocytic lesions of the iris were histologically classified as nevus or melanoma. Morphometric analysis using a digital filar micrometer (LaSICO 1602N-10 and 5-4A) measured the mean size of the 10 largest nucleoli, and Feulgen staining and image cytometry (CAS 200 Cell Analysis Systems) analyzed the nuclear DNA ploidy in the lesions. Patient follow-up i...

Microfluidic systems have significant implications in the field of in vitro cell-based assays and drug screening by virtue of their automation and high content screening capabilities. Here, we present our recent efforts to develop a microfluidic platform for high throughput and high content analysis of cell death event. A group of microfluidic devices for monitoring various cell death parameter...

This review focuses on the clinical utility and potential value of cell cycle analysis and DNA ploidy interpretation in the diagnosis of human tumors, the application of these techniques to cytologic diagnosis, and their capability for predicting disease outcome in human neoplasia. Methods of cell cycle analysis are considered, and the techniques of flow cytometry and image analysis are described.