Protein detection in complex biological fluids and matrices has become a widely diversified field utilizing a number of different technologies. The quantification of target proteins in complex media such as serum remains a challenge for most technologies such as mass spectrometry, ELISA and western blot. Quantitative Immuno-PCR has been heavily used for antigen detection in immunoassays, but mi...

Staphylococcus aureus has long been recognised as an important pathogen in human disease. Serious staphylococcal infections can frequently occur in inpatients and may lead to dire consequences, especially for therapy with antimicrobial agents. The increase in the frequency of Methicillin-Resistant Staphylococcus aureus (MRSA) as the causal agent of nosocomial infection and the possibility of em...

247 Abstract: Elevated lipoprotein(a) [Lp(a)] concentrations are associated with premature coronary heart disease and early myocardial infarction. In the general human population the sizes of apolipoprotein [apo(a)] isoforms are inversely associated with lipoprotein(a) levels. Large isoforms are associated with low Lp (a) and small isoforms with high Lp(a) in the plasma. The size of the polymop...

Alcoholic liver disease (ALD) is a global that manifests in variety of forms, including alcoholic hepatitis, inflammation, fatty liver, and cirrhosis. One the early stages ALD (AFL) disease, which primarily characterized by accumulation lipids inflammation hepatocytes. Guanylate binding protein 5 (GBP5) has been investigated for its involvement progression. The regulatory effects GBP5 on progre...

We report a case of CTX-M-55-type extended-spectrum β-lactamase (ESBL)-producing Shigella sonnei infection in a 27-year-old Korean woman who had traveled to China. The patient was admitted to the hospital due to abdominal pain, watery diarrhea, and fever (39.3℃). S. sonnei was isolated from her stool specimens, and the pathogen was found to be resistant to cefotaxime due to CTX-M-55-type ESBL. ...

Aim: Despite intensive research during the last decade, it remains challenging to prepare extracellular vesicles (EVs) of high purity, especially from primary body liquids or protein-rich conditioned media. For now, time-consuming combinations at least two orthogonal methods, e.g., density and size separation, are required enrich EVs often expense processing time. Therefore, novel technologies ...

Background: Human epidermal growth factor receptor 2 (HER2) is over-expressed in breast, ovarian, gastric, and prostate cancers used as a tumor marker the diagnosis of cancer. Monoclonal antibodies have been diagnostic therapeutic tool against HER2. Because difficulties associated with stability complexity construct high cost antibody production, we aimed to investigate, cloning, expression HER...

Gemcitabine is one of the most widely used chemotherapy drugs for advanced malignant tumors, including non‑small cell lung cancer. However, clinical efficacy gemcitabine limited due to drug resistance. The aim present study was investigate role p21 in gemcitabine‑resistant A549 (A549/G+) cancer cells. IC50 values were determined using a Cell Counting Kit‑8 (CCK‑8) assay. m...

introduction: acute leukemia is the most common malignancy in children and acute lymphoblastic leukemia (all) accounts for 75% of acute leukemia cases. new treatment protocols have resulted in complete remission rates up to nearly 100% in children with acute lymphoblastic leukemia. today, one of the most important prognostic factors in acute lymphoblastic leukemia is intensity of the treatment....