Departamento de Clínica Médica, Universidade Federal de Mato Grosso, Rua L s/ no, 78070-000 Cuiabá, MT, Brasil *Fundação Nacional de Saúde, Av. Getúlio Vargas 867, 78045-720 Cuiabá, MT, Brasil **Departamento de Virologia, Instituto Oswaldo Cruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil ***Hospital Prof. Edgar Santos, Universidade Federal da Bahia, Rua João das Botas s/ no, 40110-16...

We report here the results of the diagnostic performances of Vitros Syphilis TPA (a chemiluminescence treponemal assay) compared with those of two treponemal enzyme immunoassays and of traditional versus reverse syphilis algorithms. Ease of use, automation, and high throughput make the Vitros Syphilis TPA assay a good choice for syphilis screening in high-volume laboratories.

10 11 12 Daniel M. Musher,* Atisha Manhas, Pranav Jain, Franziska Nuila, 13 14 Amna Waqar, Nancy Logan, Bernard Marino, Edward A. Graviss, 15 16 17 From the Medical Service (Infectious Disease Section), Michael E. DeBakey 18 Veterans Affairs Medical Center, Houston, Texas, the Departments of Medicine 19 and Molecular Virology and Microbiology, Baylor College of Medicine, and the 20 Department o...

The concept of a competitive enzyme immunoassay that utilizes simultaneously the bound and the free analyte-enzyme conjugate (heterobifunctional conjugate) for signal generation in response to varying analyte concentrations in samples has been investigated. Two antigenic sites of the heterobifunctional conjugate are used in the assay for binding to immunoglobulins: the analyte derivative binds ...

Immunoassays are useful laboratory methods for clinical and forensic toxicology diagnostics. The methods are fast, sensitive, accurate and let determine poisons concentrations in different kinds of biological fluids. There are blood and urine or alternative: saliva, sweat and hair. The choice of the sample depends on the purpose of analysis. In acute poisoning the blood or serum poison concentr...

The Nezv York Times (March 25, 1962) pointed out that in the decade from 1950-60 the number of children in elementary schools had increased by fifty per cent. The prospects are for a further increase of fifty per cent during the 60's. The total cost of public education from 1950 to 1960 rose from $7 billion to $17 billion. But this is only one of the future headaches. The imagination is heardly...

Microarray technology as applied to areas that include genomics, diagnostics, environmental, and drug discovery, is an interesting research topic for which different chip-based devices have been developed. As an alternative, we have explored the principle of compact disc-based microarrays. This new methodology successfully combined high-density microarrays applied via a piezoelectric inkjet app...

"Immunoassay" defines the body of techniques which use the antibody (Ab) macromolecule, usually of the IgG class, for the detection and quantitation of an enormous range of simple and complex antigen (Ag) molecules. The success of the methods relies on both the specificity and formation constant of the Ab used, and the ability to detect the interaction between Ab and Ag. Sensitive assays in com...