A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C.

a doubtful result, the Reiter protein complementfixation (RPCF) test is performed. Sera still considered to present problems are then referred to the Treponemal Department of the National Institute of Public Health. As the two initial screening tests differ in technique but not in the nature of the antigen, one might argue for substituting one of them by a test using a treponemal antigen (Bisse...

Previous immunochemical investigations of ceruloplasmin have been primarily concerned with the assay of this substance in human sera and its localization by the technique of immunoelectrophoresis. The proteins used in the preparation of antisera appear to have been impure or poorly defined. The ready availability of crystalline human ceruloplasmin (I, 2) prompted us to study its immunochemical ...

A patient is described with myeloma without paraproteinaemia or Bence Jones proteinuria in whom the tumour cells have been shown to contain monoclonal immunoglobulin. The use of immunoelectrophoresis and polyacrylamide gel electrophoresis has established, apparently for the first time, that the immunoglobulin components are present in the form of intact molecules and free light chains.