Modern Confocal Laser Scan Microscopy is a sophisticated technique allowing acquisition of information from different fluorescence markers in the same tissue by separating them into different confocal channels. For an adequate interpretation of these multidimensional images advanced image processing techniques are required. In this study, we introduce an automated image analysis based on Boolea...

background: aflatoxin m1 (afm1) is a toxic and carcinogenic mycotoxin which after presence in milk and dairy products such as cheese and butter could make them as contaminated food. the main objective of this study was to evaluate the concentration of afm1 in pasteurized and traditional cheese marketed in southern khorasan, from december 2011 to january 2012. methods: a total of 102 cheese samp...

Modern Confocal Laser Scan Microscopy is a sophisticated technique allowing acquisition of information from different fluorescence markers in the same tissue by separating them into different confocal channels. For an adequate interpretation of these multidimensional images advanced image processing techniques are required. In this study, we introduce an automated image analysis based on Boolea...

Johne’s disease or paratuberculosis is a chronic mycobacterial infection that affects cattle, sheep, goats and other ruminants, adversely, leading to huge economic losses throughout the world. The estimation of sero-prevalence of this disease in the cattle population of south-western Bangalore, Karnataka, using an immunological assay and statistical analyses, was the objective of this study. On...

To establish a sensitive method to detect IgA Fc receptors (Fc alpha R) on human and murine lymphoid cells, fluorescent microspheres (FMS) were used in a flow cytometric assay. The following three assays with FMS were tested and compared with a conventional indirect Fc alpha R assay using FITC-labeled anti-IgA: (1) direct cell binding assay with murine myeloma IgA(MOPC315)-coated FMS(IgA-FMS as...

sera from ninety one patients clinically suspected to extra intestinal amoebiasis (ex-am) were collected and examined for presence of anti entamoeba histolytica (e.h) antibody. the tests were indirect immunofluorescence antibody, (ifa or iif), gel diffusion (gd) and counter immuno electrophoresis (ciep). thirty one patients (34%) had anti e.h. antibody titres of >1:80. among them eight patients...