Diaspirin cross-linked haemoglobin (DCLHb) is a new oxygen carrying blood substitute with vasoactive properties. Vasoactive properties may be mediated via high affinity binding of nitric oxide by the haem moiety. Using a rodent model of head injury combined with ischaemia, we studied the effects of DCLHb on cerebral blood flow (CBF) and intracranial pressure (ICP). Twenty anaesthetized rats wer...

The Agilent 7500c Inductively Coupled Plasma Mass Spectrometer (ICP-MS) was used for the fast, routine, and reliable analysis of 23 trace elements in urine. The complete method validation for all elements is described, including the evaluation of short-term and long-term stability, the analysis of different reference materials and the discussion of precision and accuracy in internal and externa...

An ICP-MS validated method for the rapid determination of chromium (Cr), selenium (Se), and molybdenum (Mo) in infant formula and adult nutritional products was successfully transferred from the Agilent 7500cx ICP-MS to the Agilent 7700x ICP-MS, and from lab 1 to lab 2, respectively. The key advantage of the method is that all samples can be analyzed using a single-cell gas mode (helium mode) t...

The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins werefractionated using SDS-PAGE. Results showed statistically significant differences ...

brucellosis, caused by brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. among brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. in this study, the immunogeni...

An Arg345Trp (R345W) mutation in epidermal growth factor-containing, fibulin-like extracellular matrix protein 1 (EFEMP1) causes its inefficient secretion and the macular dystrophy malattia leventinese/Doyne honeycomb retinal dystrophy (ML/DHRD). To understand the influence of the protein homeostasis (or proteostasis) network in rescuing mutant EFEMP1 misfolding and inefficient secretion linked...

Abstract The T cell response is an important detection index in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine development. present study was undertaken to determine the epitopes spike (S) protein of SARS-CoV-2 that dominate responses SARS-CoV-2–infected patients. PBMCs from rhesus macaques vaccinated with a DNA encoding full-length S were isolated, and ELISPOT assay used ...

objective(s): streptokinase (ska) is an antigenic protein which is secreted by streptococcus pyogenes. streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. in the present study, recombinant streptokinase from s. pyogenes was produced and showed that recombinant ska protein was recognized by infected human sera using western blot analysi...

Abstract Background Both the highly pathogenic avian influenza (HPAI) H5N1 and low (LPAI) H9N2 viruses have been reported to cross species barriers infect humans. can cause severe damage are associated with a high mortality rate, but do not such outcomes. Our purpose was use proteomics technology study differential expression of mitochondrial-related proteins related virus infections. Methods A...

BACKGROUND The hepatitis C virus (HCV) Alternate Reading Frame Protein (ARFP or F protein) presents a double-frame shift product of the HCV core gene. We and others have previously reported that the specific antibodies against the F protein could be raised in the sera of HCV chronically infected patients. However, the specific CD4(+) T cell responses against the F protein during HCV infection a...