The putative viral transcriptase p90 in infectious bursal disease virus (IBDV) was shown to form an enzyme-guanylate intermediate which is indicative of guanylyl-transferase activity. The p90-nucleotide bond is most likely to be a phosphodiester linkage, as it resisted treatment with HCl and NH2OH but was sensitive to NaOH. This is in contrast to phosphoamide bonds formed by reovirus cores. Met...

to detect the presence of infectious bronchitis virus (ibv) in infected allantoic fluid (af) of spf embryonated eggs rapid hemagglutination (ha) activity after treatment with neuraminidase enzyme was used. twenty ibv suspected materials were inoculated in spf embryonated eggs via chorioallantoic cavity. harvested afs were treated with neuraminidase enzyme and the presence of ibv was detected by...