Propanol appeared better than ethanol for the long-term storage of spinach leaves, followed by hexanol and pentanol. The lowest molecular weight and yields of DNA came from spinach stored in methanol and ethanol. In an experiment with spinach leaves stored in 100, 95, 70, 50 and 25% ethanol, the 50% ethanol stored leaves appeared to yield more and higher molecular weight DNA than any other trea...

Campylobacter jejuni has been isolated previously from market produce and has caused gastroenteritis outbreaks linked to produce. We have tested the ability of this human pathogen to utilize organic compounds that are present in leaf and root exudates and to survive in the plant environment under various conditions. Carbon utilization profiles revealed that C. jejuni can utilize many organic ac...

Our knowledge on the absorption of folate is incomplete. The deconjugation process as a possible limiting factor in the absorption of folates was investigated. The study also attempted to validate the use of the area under the serum response curve (AUC) from food compared with folic acid as a proxy variable for food folate bioavailability. Folate absorption was determined in healthy ileostomy v...

Quarantine inspectors search for fruit fly infestations in incoming shipments by visual inspection and by dissecting or cutting a sample of fruit in each shipment. The reliability of the latter procedure for detecting fruit fly larvae is questionable and, therefore, a test was conducted to determine its effectiveness. Infested grapefruit, mangoes, guavas, and carambolas were cut open to determi...

We designed an in vitro signal amplification circuit that takes a short RNA input that catalytically activates the Spinach RNA aptamer to produce a fluorescent output. The circuit consists of three RNA strands: an internally blocked Spinach aptamer, a fuel strand, and an input strand (catalyst), as well as the Spinach aptamer ligand 3,5-difluoro-4-hydroxylbenzylidene imidazolinone (DFHBI). The ...

We examined the physiological and biochemical responses of resistant ('Halt' and 'Prairie Red') and susceptible ('TAM 107') wheat, Triticum aestivum L., to injury by the Russian wheat aphid, Diuraphis noxia (Mordvilko). Photosynthetic capacity was evaluated by measuring assimilation/internal CO2 (A/Ci) curves, chlorophyll fluorescence, chlorophyll, and nonstructural carbohydrate content. Total ...

Infestations by the weedy perennial plant Lantana camara L. (lantana) cause major disruptions to biodiversity; however, the effects of lantana on soil fungal communities are poorly understood, yet may interrupt important mutualisms in natural and semi-natural ecosystems. This research is aimed at understanding the impacts of lantana on the soil fungal community of five sites on contrasting soil...

Honey bees (Apis melliferaL.) (Hymenoptera: Apidae) have been selectively bred for varroa-sensitive hygiene (VSH), which is the removal of pupae that are infested byVarroa destructor Anderson&Trueman fromcappedbrood cells. This hygienic behavior is a complex interactionof bees and brood inwhich brood cells are inspected, and then brood is either removed or recapped. Previous work has shown that...

Abstract The dissipation of ethoprophos and tricyclazole in soil their translocation tendency to spinach were investigated. Prior field trials, the analytical method for determination these pesticide residues was optimized validated on spinach. trial conducted under greenhouse conditions two different pretreatment periods with pesticides. After treating pesticides 30 (PBI-30) 60 days (PBI-60) b...