Based on RFLP analysis, two evolutionary lineages for Brassica diploid species have been proposed. These are (I) the “nigra” lineage and (II) the “rapa/oleracea” lineage. The phylogenetic relationship of Raphanus species to these two lineages is still unclear because chloroplast and mitochondrial DNA genomic restriction site variation suggests that Raphanus is more closely related to the “rapa/...

Flavobacterium columnare is an important pathogen of freshwater fish, implicated in skin and gill disease, often causing high mortality. An outbreak of skin disease in fingerling and adult Nile tilapia, Oreochromis niloticus (L.), cultivated in a recirculation system, was investigated. Four strains were isolated and characterized by biochemical reactions, enzyme production, fatty acid profile a...

UNLABELLED PREMISE OF THE STUDY New primers were developed for Bignonieae to enable phylogenetic studies within this clade using herbarium samples. • METHODS AND RESULTS Internal primers were designed based on available sequences of the plastid ndhF gene and the rpl32-trnL intergenic spacer region, and the nuclear gene PepC. The resulting primers were used to amplify DNA extracted from her...

Sinorhizobium meliloti and Sinorhizobium medicae are two closely related species of the genus Sinorhizobium showing a similar host range, nodulating leguminous species of the genera Medicago, Melilotus and Trigonella, but their phylogenic relationship has not been elucidated yet. In this paper we report the application of three different molecular markers, (i) RFLP of nodD genes, (ii) 16S-23S r...

The molecular diversity among 60 isolates of Renibacterium salmoninarum which differ in place and date of isolation was investigated by using randomly amplified polymorphic DNA (RAPD) analysis. Isolates were grouped into 21 banding patterns which did not reflect the biological source. Four 16S-23S rRNA intergenic spacer (ITS1) sequence variations and two alleles of an exact tandem repeat locus,...

References 1. Niu Q, Guan G, Yang J, Fu Y, Xu Z, Li Y, Miling Ma1, Liu Z, Liu J, Liu A, Ren Q, Jorgensen W, Luo J, Yin H: Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China. BMC Veterinary Research 2011, 7:17. 2. Rijpkema SG, Molkenboer MJ, Schouls LM, Jongejan F, Schellekens JF: Simultaneous Detection and Genotyping of Three Genom...

To examine whether microbial community structure differs across rice genotypes, automated ribosomal intergenic spacer analysis (ARISA) was conducted. Nine cultivars of Oryza sativa ssp. indica or japonica and seven lines of other Oryza species were grown in paddy fields with low, standard, and high levels of N fertilization. Multidimensional scaling plots of bacterial ARISA for aerial parts of ...

The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All seven strains showed the cultural and biochemical properties typical of A. haemolyticum and were susceptible to most of the antibiotics tested. The species identification could be confirmed by amplification and sequencing of the 1...

An overview on legal regulation, taxonomy, and botanical and chemical methods of analysis of marijuana are provided. Emphases are placed on the applications of the recently developed molecular biology methods to the identification and characterization of marijuana samples. Procedures for the extraction of plant DNA, DNA amplification, restriction analysis, and sequence analysis are reviewed. Th...

Fusarium isolates from highland areas in Malaysia were mostly recovered two species of grasses, Elyhordeum montanense and Paspalum conjugatum. The grouped into four morphological groups. Based on TEF-1α sequences, morphotype 1 molecularly identified as F. graminearum complex, 2 venenatum, 3 avenaceum 4 kyushuense. Restriction analysis the Intergenic Spacer region showed high levels genetic dive...