Automated rRNA intergenic spacer analysis (ARISA) was used to characterise bacterial (B-ARISA) and fungal (F-ARISA) communities from different soil types. The 16S-23S intergenic spacer region from the bacterial rRNA operon was amplified from total soil community DNA for B-ARISA. Similarly, the two internal transcribed spacers and the 5.8S rRNA gene (ITS1-5.8S-ITS2) from the fungal rRNA operon w...

The two internal transcribed spacers (ITS1 and ITS2) of rDNA of three members of the Ixodes ricinus "complex" (Acari: Ixodidae) were sequenced. Sequence variation was assessed for the North American species I. scapularis, I. dammini, and I. pacificus at three levels: within individual/population, between individuals of different geographic origin within a species, and between species. Both spac...

We examined the relative merits of mitochondrial DNA loci and ribosomal DNA internal transcribed spacers for their use in prospecting for cryptic species of platyhelminth parasites. Sequence divergence at ITS1 and ITS2 was compared with divergence at 2 mtDNA loci (NADH dehydrogenase-1 and cytochrome c oxidase I) between closely related species of trematodes and cestodes. Both spacers accumulate...

The clustered regularly interspaced short palindromic repeat (CRISPR) confers adaptive immunity against phages via sequence fragments (spacers) derived from mobile genetic elements (MGEs), thus serving as a memory of past host-phage co-evolution. To understand co-evolutionary dynamics in natural settings, we examined CRISPR diversity in 94 isolates of Microcystis aeruginosa from a small eutroph...

Ribosomal DNA sequence data has been important in elucidatingmolecular evolution, discerning phylogenetic relationships, and in developing species diagnostics. Species in the genus Anastrepha Schiner, particularly members of the Fraterculus Species Group, are considered the most important native pests of fruit in the New World. This study presents the Þrst complete rDNA sequence data spanning t...

Various molecular techniques like analysis of the amplified rDNA internal transcribed spacers (ITS), intragenic spacers and total ITS region analysis by restriction fragment length polymorphism (RFLP) has been introduced for yeast identification but there are limited databases to identify yeast species on the basis of 5.8S rDNA. In this study, twenty nine yeast strains from various sources incl...

BACKGROUND: Dahlia tuber as the source of isolates in endophytic mold produces Inulinases. Inulinase is used industry to produce inulin become fructose through an enzymatic reaction. Fructose from natural substances constitutes low calories diet diabetes mellitus type 2 patients. An important phase done obtain inulinase enzyme isolation. AIM: The objective this research was isolate and identify...

We determined the nucleotide sequence of a D. melanogaster ribosomal DNA spacer. Sequences of various portions of different cloned ribosomal spacers have been previously reported. We extend the analysis to cover the entire nontranscribed and external transcribed regions. Comparison to other cloned ribosomal DNA gene units of this species confirms a conserved general organization of the ribosoma...

Abstract Background Wild-type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has be determined. In this study, wild-type ethanologenic isolated from a secondary effluent were assessed capability ferment saccharified microalgae sugars. Results Yeast species in wastewater identified sequenci...

Abstract The research is devoted to the analysis of biodiversity Antarctic cyanobacteria in hypolithic organic-accumulative horizons soils Larsemann Hills oasis. Studies fouling glasses by methods light and confocal microscopy, as well fluorescent situ hybridization, indicate that filamentous forms predominate among upper layers microbial communities. Strains genera Nostoc , Halotia Leptolyngby...