Intradermal administration of naked DNA with a conventional needle syringe is simple and inexpensive method to expose an encoded antigen the dermal immune system. We aimed enhance intradermal gene expression pyro-drive jet injector using pig skin, which similar in structure biomechanical properties human skin. When Cy3-labeled plasmid (pCy3) was applied skin injector, pCy3 distributed preferent...

BACKGROUND Subcutaneous immunotherapy with high dose grass pollen (typically microgram quantities) was first described over 100 years ago. This treatment suppresses allergen-induced cutaneous late responses, with lesser effects on early responses. We previously reported that repeated 2-weekly intradermal injections of grass pollen - containing approximately 7 ng of major allergen Phl p 5 - led ...

sentinel lymph node is the first regional lymph node that drains the lymph from the primary tumor. it is potentially the first nodeto receive the seeding of lymph‑borne metastatic cells. this study aimed to discuss lymphoscintigraphy procedural guidelines fordetection of sentinel node using tc‑phytate in isfahan, iran. moreover, the preliminary results of the first year’s clinical experience of...

The need for test systems for nanoparticle biocompatibility, toxicity, and inflammatory or adaptive immunological responses is paramount. Nanoparticles should be free of microbiological and chemical contaminants, and devoid of toxicity. Nevertheless, in the absence of contamination, these particles may still induce undesired immunological effects in vivo, such as enhanced autoimmunity, hypersen...

BACKGROUND Imiquimod, a synthetic Toll-like receptor 7 agonist enhanced immunogenicity of influenza vaccine in a mouse model. We hypothesized that topical imiquimod before intradermal influenza vaccination (TIV) would produce similar effect in human. METHODS We performed a prospective 1-year follow-up, double-blind, randomized, controlled trial with adults with comorbidities. Participants wer...

Tuberculin skin test (TST), an age old method is based on measuring delayed-type hypersensitivity (DTH) response to purified protein derivative (PPD). However, inspite of simplicity, ease and cost effectiveness, the usefulness of PPD test is limited due to its inability to distinguish among a protective immune response, latent infection and active tuberculosis disease. On the other hand, a skin...

A 16-year-old-girl with a past medical history of lidocaine anaphylaxis requested an allergy test to find a local anesthetic (LA) agent with no adverse allergic reactions for her ingrown toenail treatment. A skin prick test (SPT) and intradermal test were administered using lidocaine, procaine, bupivacaine, mepivacaine and ropivacaine. All LA agents used in the SPT showed negative result. An in...

Paratuberculosis vaccination in cattle has been restricted due to its possible interference with the official diagnostic methods used in tuberculosis eradication programs. To overcome this drawback, new possibilities to detect Mycobacterium bovis infected cattle in paratuberculosis vaccinated animals were studied under experimental conditions. Three groups of 5 calves each were included in the ...

Chlorpheniramine is a widely prescribed H1-antihistamine for relieving urticaria or histamine-mediated allergic reactions. However, although rare, it may cause immediate hypersensitivity reactions. The diagnosis is usually made by provocation test, but its application is often limited due to comorbidities or potential risk of severe reactions. In those cases, skin tests and basophil activation ...

Oral immunization of healthy adults with 10(7) CFU BCG Moreau Rio de Janeiro was well tolerated and significantly boosted gamma interferon responses to purified protein derivative, Ag85, and MPB70 from previous childhood intradermal BCG immunization. Oral BCG offers the possibility of a needle-free tuberculosis vaccine and of boosting the protective immunity from intradermal tuberculosis vaccines.