Drosophila simulans and Drosophila mauritiana are closely related sibling species, the former cosmopolitan and the latter restricted to the small oceanic island of Mauritius. Genetic analysis of male sterility in hybrids between these species shows that at least five loci (one on each chromosome arm) are responsible for this reproductive isolation. This is the most loci that could have been det...

The full coding sequences of two novel human enterovirus (HEV)-C serotypes 105 and 116, sampled in the Republic of the Congo in 2010 and in Russia in 2011, were identified in this study. Enterovirus (EV)-105 was closest to EV-104 in the 5' NTR and to EV-109 in the coding genome region. It had the same unconventional 5' NTR as EV-104 and EV-109. The non-cytopathogenic EV-116 was phylogenetically...

The application of heterosis (hybrid vigor) has brought great success to plant breeding, particularly of hybrid rice, achieving significant yield increases. Attempts to explore the heterosis of inter-subspecific hybrids between indica and japonica rice, which result in even greater yield increases, have greatly increased in the past decades. However, because of the reduced seed setting rate in ...

Species barriers, expressed as hybrid inviability and sterility, are often due to epistatic interactions between divergent loci from two lineages. Theoretical models indicate that the strength, direction, and complexity of these genetic interactions can strongly affect the expression of interspecific reproductive isolation and the rates at which new species evolve. Nonetheless, empirical analys...

There is great demand for flexible biomolecule analysis platforms that can precisely quantify very low levels of multiple targets directly in complex biological samples. Herein we demonstrate multiplexed quantification of microRNAs (miRNAs) on encoded hydrogel microparticles with subfemtomolar sensitivity and single-molecule reporting resolution. Rolling circle amplification (RCA) of a universa...

The evolution of reproductive barriers is of central importance for speciation. Here, we investigated three components of postzygotic isolation-embryo mortality, hybrid inviability, and hybrid sterility-in a group of food-deceptive Mediterranean orchids from the genera Anacamptis, Neotinea, and Orchis. In these orchids, pollinator-mediated isolation is weak, which suggests that postpollination ...

The detection of Legionella pneumophila in water via guinea-pig intraperitoneal injection has been compared with direct isolation of these organisms on semiselective BCYE alpha media. Both techniques were of similar sensitivity, detecting as few as 10(2) L. pneumophila in 10 ml of water. However, at this concentration, detection using guinea-pig intraperitoneal injection was via the indirect pa...

Wind turbines are subject to variable aerodynamic loads and extreme environmental conditions. Wind turbine components fail frequently, resulting in high maintenance costs. For this reason, gearbox condition monitoring becomes important since gearboxes are among the wind turbine components with the most frequent failure observations. The major challenge here is the detection of faults under the ...

Speciation is fundamental to the process of generating the huge diversity of life on Earth. However, we are yet to have a clear understanding of its molecular-genetic basis. Here, we examine a computational model of reproductive isolation that explicitly incorporates a map from genotype to phenotype based on the biophysics of protein-DNA binding. In particular, we model the binding of a protein...

Monitoring of the air intake system of an automotive engine is important to meet emission related legislative diagnosis requirements. In this paper, the problem of fault detection in the air intake system is stated as a constraint satisfaction problem over continuous domains with a big number of variables and constraints. This problem can be solved using Consistency Techniques. Consistency tech...