The intracellular localization of the activity and synthesis of three isozymes of NAD(P)(+)-glutamate dehydrogenase from the unicellular green alga Chlamydomonas reinhardtii cw-92 has been established. Isozyme activities have been located within mitochondria by using differential centrifugation techniques and discontinuous Percoll gradient separations. Experiments with protein synthesis inhibit...

Coordination compounds of 5-chloroacetamido-1,3,4-thiadiazole-2-sulfonamide (Hcaz) with V(IV), Cr(lll), Fe(ll), Co(ll), Ni(ll) and Cu(ll) have been prepared and characterized by standard procedures (spectroscopic, magnetic, EPR, thermogravimetric and conductimetric measurements). Some of these compounds showed very good in vitro inhibitory properties against three physiologically relevant carbo...

Abstract—Genetic diversity in plant materials used for revegetation of disturbed sites is important to allow natural selection and adaptation to occur. Several methods for estimation of genetic diversity using molecular markers are available. Protein markers, such as isozymes and seed storage proteins, have been used extensively, but their use has declined in recent years in favor of DNA marker...

Four laccase isozymes (LCC1, LCC2, LCC3 and LCC4) synthesized by Pleurotus ostreatus strain V-184 were purified and characterized. LCC1 and LCC2 have molecular masses of about 60 and 65 kDa and exhibited the same pI value (3.0). Their N termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. Laccases LCC3 and LCC4 were characterized b...

HE detection of bands of enzyme activity showing different rates of migration Tduring starch gel electrophoresis has been reported repeatedly in recent years. This technique has been particularly useful in genetic studies, and many cases are now known of a band or bands, termed isozymes, which are under genetic control. Such control has been demonstrated for alkaline phosphatase in bacteria ( S...

A combined histochemical, biochemical and electrophoretic study with respect to the enzymes succnic dehydrogenase(SDH), myofibrillar adenosine triphosphatase (m-ATPase), lactate dehydrogenase (LDH) isozymes and myosin light chains was carried out to investigate the response of rat gastrocnemius muscle (medial head). Twelve weeks after thyroidectomy, the results indicated a shift from fast to sl...

The lactate dehydrogenases commonly occur in from one to five molecular forms (isozymes) in the various tissues of an animal species.' By means of zone electrophoresis, characteristic patterns of these isozymes may be demonstrated in each tissue. In adult animals, most tissues contain all five isozymes but in different proportions. Usually, heart muscle exhibits principally the first, or most n...

The recognition of protein kinase C (PKC) as the long soughtafter receptor for the tumor-promoting phorbol esters established the potential role of PKC in carcinogenesis and as a potentially important target for cancer therapeutics (1, 2). PKC is a family of serine/threonine kinases that regulate a variety of cell functions including proliferation, gene expression, cell cycle, differentiation, ...

The development of human benign or malignant prostatic diseases is closely associated with androgens, primarily testosterone (T) and dihydrotestosterone (DHT). T is converted to DHT by 5-alpha reductase (5-AR) isozymes. Differential expression of 5-AR isozymes is observed in both human benign and malignant prostatic tissues. 5-AR inhibitors (5-ARI) are commonly used for the treatment of benign ...

The more acidic, minor isozymes of human carbonic anhydrase types B and C are present in higher concentrations in the older erythrocyte populations. The levels of all of these isozymes can be determined quantitatively by immunochemical methods following their separation by electiofocusing. A wide variety of human tissues have been assayed for carbonic anhydrases. Significant levels of the C typ...