Conflicting findings regarding proadhesion and antiadhesion in cell-to-cell interactions were previously reported for CD43. We examined possible differences in the role of the 130-kd glycoform and the 115-kd glycoform of CD43 in cellular adhesion in vitro. We generated a monoclonal antibody (MFT3) that discriminates between helper and nonhelper murine T-cell clones. Characterization of MFT3 wit...

Loss of gastric acid secretion is pathologically known as achlorhydria. Acid-secreting parietal cells are characterized by abundant expression of ezrin (Vil2), one of ezrin/radixin/moesin proteins, which generally cross-link actin filaments with plasma membrane proteins. Here, we show the direct in vivo involvement of ezrin in gastric acid secretion. Ezrin knockout (Vil2(-/-)) mice did not surv...

Affinity-purified antibodies to mouse liver 26- and 21-kD gap junction proteins have been used to characterize gap junctions in liver and cultured hepatocytes. Both proteins are colocalized in the same gap junction plaques as shown by double immunofluorescence and immunoelectron microscopy. In the lobules of rat liver, the 21-kD immunoreactivity is detected as a gradient of fluorescent spots on...

in the present study, we investigated total cell protein patterns of ten isolates of epidermophyton floccosum var.pigmented by sds-page on 10% polyacrylamide resolving gels. densitometric analysis of the gels allowed to detect more than 31 clearly detectable mycelial protein bands with molecular weights in the range of 12 to 98 kd proteins of 12.5, 13.5, 14.4, 16, 18.4, 19.7, 20.1, 23.5, 26, 27...

In the present work, we investigate solar radiation climate of Saudi Arabia, using data from 43 sites in country covering period 2013–2021. These include hourly values global, G, and diffuse, Gd, horizontal irradiances which direct, Gb, irradiance is estimated. The diffuse fraction, kd; direct-beam kb; ratio ke = Gd/Gb, are used analysis. Solar maps annual mean kd, kb, prepared for Arabia under...

Abstract The partial least squares (PLS) is a commonly applied multi-variate method in anomaly detection problems. PLS strategy has been amalgamated with $$T^{2}$$ T 2 and squared prediction error (SPE) based statistical indicators to detect anomalies process. These tra...

Penelitian ini menerapkan pengendali Proporsional Integral Derivatif (PID) untuk mengendalikan kecepatan sudut motor DC dan tuning Ziegler Nichols. tidak hanya terbatas simulasi menggunakan Matlab namun juga implementasi ke perangkat keras Arduino. Pengendali PID banyak digunakan karena sederhana mudah dalam pengaplikasiannya. Namun terdapat kekurangan menentukan nilai parameter kontroler atau ...

To identify proteins associated with nicotinic postsynaptic membranes, mAbs have been prepared to proteins extracted by alkaline pH or lithium diiodosalicylate from acetylcholine receptor-rich (AChR) membranes of Torpedo electric organ. Antibodies were obtained that recognized two novel proteins of 87,000 Mr and a 210,000:220,000 doublet as well as previously described proteins of 43,000 Mr, 58...

RATIONALE Kawasaki disease (KD), an acute febrile vasculitis, is the most common cause of acquired heart disease in childhood; however, diagnosing KD can be difficult. OBJECTIVE To identify unique proteomic biomarkers that can be used to facilitate earlier diagnosis of KD. METHODS AND RESULTS We enrolled 214 children with fever and clinical features suggestive of KD. Of those, only 100 were...

The major concanavalin A (Con A)-binding component in urea/deoxycholate/mercaptoethanol extracts from pig ear epidermis had an apparent Mr of 78 kD. In indirect immunofluorescence affinity-purified polyclonal antibodies against this glycopolypeptide strongly stained the surface of suprabasal cells in the epidermis of pig and human skin. Immunocytochemical labeling with gold-labeled second antib...