Individual colonies of micrococcus euryhalis and of a marine bacterial isolate were grown in pour tubes under hydrostatic pressure. The medium was prepared in a silica sol, and gelation was effected at 4 degrees C by addition of salts to achieve concentrations found in seawater.

Feeding deterrents or antifeedant chemicals are those chemicals which inhibit feeding of insect on a treated food material without necessarily killing or repelling them. Plants with ant feeding properties are increasingly being used against phytophagous insect pests for protection of crops. In the present study of growth regulating action of Leaf extract of Catharanthus roseus on development st...

Lysozyme production is a frequent property of staphylococcal strains isolated from various sources; all 503 tested strains of Staphylococcus aureus and 13 out of 35 strains of Staphylococcus epidermidis produced an enzyme lysing Micrococcus lysodeikticus as tested by a modified plate method. Lysozyme production by staphylococci is more frequent than the production of free coagulase, clumping fa...

The accuracy of lysozyme concentration determination by the method of measuring lysis diameter in agarose gel slabs containing Micrococcus lysodeikticus bacteria uniformly suspended throughout the gel was determined for various methods of tear sample collection. The effects of storage of lysozyme solution samples at subzero temperatures for several days was also examined. It was found that a po...

Anti-phosphatidylglycerol and anti-cardiolipin antisera were prepared in rabbits by using phospholipids purified from Micrococcus lysodeikticus. Anti-phosphatidylglycerol antibodies were found in antisera when either phosphatidylglycerol or cardiolipin were used as immunogens, but adsorption studies indicated they were not similar. Antibodies which reacted with phosphatidylinositol and phosphat...

The moderately halophilic bacterium Micrococcus varians, isolated from soy sauce mash, produced extracellular nuclease when cultivated aerobically in media containing 1 to 4 M NaCl or KCl. The enzyme, purified to an electrophoretically homogeneous state, had both ribonuclease and deoxyribonuclease activities. The nuclease had maximal activity in the presence of 2.9 M NaCl or 2.1 M KCl at 40 C. ...

The HEW lysozyme (Lys) and the fac-[Re(CO)(3)(H(2)O)(3)](+) complex (1) are used as a simple model system for the description of a new approach to the labelling polypeptides with fluorescent tags. The strategy takes advantage of the reaction of an acridine orange-based fluorophore (AO) with the non-native metal fragment 1 hybridized on the enzyme. A synthetic methodology for the quantitative me...

Some observations on the ability of washed Escherichia coli and Mficrococcus lysodeikticus to form colonies when plated after having been freeze-dried and exposed to water vapor in vacuo will be presented in this paper. Serratia marcescens treated in this manner is rapidly killed within a rather narrowly limited range of relative humidities around 90 per cent (Monk and McCaffrey, 1957). It was ...

A total of 221 strains of staphylococci and 98 strains of micrococci isolated from the skins of Eastern gray squirrels, Southern flying squirrels, raccoons, opossums, squirrel monkeys, swine, sheep, horses, cattle, and dogs were characterized in a preliminary attempt to resolve their natural relationships and distribution in nature. Staphylococci demonstrating the widest host range included Sta...