The aim of the present study was to evaluate the imipenem-resistant mechanism via the outer membrane protein (OMP) OprD2 and metal β-lactamase expression in the cell wall of Pseudomonas aeruginosa. The Pseudomonas aeruginosa was clinically separated and validated by VITEK-2 full-automatic bacteria analyzer. Drug resistance, sensitive antibiotics and minimum inhibitory concentration (MIC) were t...

The origin of carbapenem-hydrolyzing metallo-β-lactamases (MBLs) acquired by clinical bacteria is largely unknown. We investigated the frequency, host range, diversity, and functionality of MBLs in the soil microbiota. Twenty-five soil samples of different types and geographical origins were analyzed by antimicrobial selective culture, followed by phenotypic testing and expression of MBL-encodi...

Abstract Background Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infection (UTI); however, treatment UTI has been challenging due to increased antimicrobial resistance (AMR). One the most important types AMR carbapenem (CR). CR bacteria are known as an threat global public health today. Class B metallo-beta-lactamases (MBLs) one factors for against carbapenems. We aim...

OBJECTIVES The aim of the study was the biochemical characterization of a new variant of the metallo-beta-lactamase, IMP-22. Moreover, the genetic environment of the bla(IMP-22) gene was investigated in Pseudomonas fluorescens and Pseudomonas aeruginosa collected from urban wastewater and a teaching hospital in L'Aquila, Italy. METHODS Molecular characterization of genetic elements was carrie...

The clinical impact of β-lactamase has become a public health problem around the world in terms increased morbidity and mortality, especially child population. This study was aimed at determining bacteriological profile detection producing bacteria isolated from blood samples neonates. For this study, total 1335 neonates admitted NICU, SCBU, sepsis-suspected visiting Paropakar Maternity Women’s...

BACKGROUND Peritoneal dialysis (PD) is an established treatment modality for end-stage renal disease (ESRD). Peritonitis remains a serious complication in PD patients and an important cause of drop-out from the program. Types of pathogens and their drug resistance patterns may determine the outcome of peritonitis. The present study was undertaken to determine the microbiology of peritonitis in ...

Enterobacteriaceae clinical strains that produce the class A carbapenem-hydrolysing enzyme KPC (Klebsiella pneumoniae carbapenemase) are increasingly reported worldwide, and are already endemic in North and South America, China, Israel and Greece. The accurate detection of KPC enzymes is of utmost importance for containing the global spread of KPC producers. Currently, the detection of putative...

igh-level carbapenem resistance in Pseudomonas aeruginosa s a recognized public health problem. Metallo-lactamase MBL) is one of the most common resistance mechanisms n these microorganisms. The gene blaSPM-1, which is the ost frequent metallo-lactamase gene in Brazil, was first escribed in 20021 and has been found in different regions.2 ntil now there has been no record of carbapenemase enzyme...

Growing incidences of β-lactamase producing uropathogens have been observed worldwide. Extended Spectrum β-lactamase (ESBLs) and Metallo β-lactamase (MBLs) are two types of β-lactamases that have emerged as a major threat with limited treatment options. In the current study, the effect of Avicennia marina leaf and stem extracts was studied on 10 ESBL and 10 MBL producing uropathogens which were...