The preparation of gold nanomolecules with sizes other than Au(25)(SR)(18), Au(38)(SR)(24), Au(102)(SR)(44), and Au(144)(SR)(60) has been hampered by stability issues and low yields. Here we report a procedure to prepare Au(67)(SR)(35), for either R = -SCH(2)CH(2)Ph or -SC(6)H(13), allowing high-yield isolation (34%, ~10-mg quantities) of the title compound. Product high purity is assessed at e...

T detection and identification of molecules within complex biological matrixes (i.e., plant tissue) requires homogenization, filtration, and liquid extraction of the sample to prepare for analysis using techniques such as gas chromatography/mass spectrometry (GC/MS), liquid chromatography-mass spectrometry (LC-MS), capillary electrophoresis-mass spectrometry (CE-MS), and LC-NMR. Direct analysis...

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous ...

While non-native protein conformations such as folding intermediates are rarely observed in solution such species are often stabilized as gaseous ions during electrospray ionization for mass spectrometry. This opens the possibility of large scale efforts to capture information about many non-native structures such as folding intermediates or malformed conformations having deleterious effects: s...

A simple method for frequency locking a frequency-doubled Nd:YAG laser to the center of line 1109 of the iodine absorption spectrum is described. The 31.6 GHz frequency shift provided by stimulated-Brillouin scattering from a single-mode silica fiber provides a probe signal that lies on the edge of line 1105 of the iodine spectrum. We adjust the frequency of the laser to maintain the transmissi...

Ultra-high luminance lamps emitting white light with a well-scattered blue spectrum from InGaN/GaN laser diodes and a phosphor-converted yellow spectrum show speckle contrast values as low as LED. Spectral behavior of the laser diodes is analyzed to find the reason why such low values are obtained. As a result, the PWM-driven, multilongitudinal mode with dynamically broadened line-width is foun...

An exact result for the spectral density of intensity variations that occur after propagation of ergodic light in a medium having lowest-order-only group-velocity dispersion is obtained and applied to the problem of semiconductor laser phase noise to intensity noise conversion in a single-mode optical fiber. It is shown that the intensity spectrum after propagation formally approaches, for a la...

A computer controlled cw UV-visible dye laser source for spectroscopic use has been developed. Computer control facilitates both continuous single-frequency scanning and data acquisition. With 4 W of Ar-ion laser pump power, such a spectrometer can generate in excess of 1 W of cw single-frequency power in the visible and up to 1 mW in the UV by using extracavity nonlinear optical mixing. The la...

We have developed an algorithm called Q5 for probabilistic classification of healthy versus disease whole serum samples using mass spectrometry. The algorithm employs principal components analysis (PCA) followed by linear discriminant analysis (LDA) on whole spectrum surface-enhanced laser desorption/ionization time of flight (SELDI-TOF) mass spectrometry (MS) data and is demonstrated on four r...