In the present study, we report the design and synthesis of peptide-based-multi-walled carbon nanotubes (MWCNTs) to target mitochondria. Targeting these intracellular organelles might open the way to develop alternative systems to address diseases related to genetic mutations in mitochondrial (mt)-DNA, by delivering therapeutic oligonucleotides. The first step towards mitochondrial delivery of ...

In this paper and video, we describe the protocols used in our laboratory to study the targeting preferences of regenerating cell processes of adult retinal neurons in vitro. Procedures for preparing retinal cell cultures start with the dissection, digestion and trituration of the retina, and end with the plating of isolated retinal cells on dishes made especially for use with laser tweezers. T...

The advent of the Ultraintense Tabletop Laser Systems (UITLSs) has opened new and until then unexpected frontiers allowing the development and the progress of new research areas, in particular in scientific and industrial fields. Usually, these laser systems are used to generate plasma on solid or gaseous targets, so-called Laser Induced Plasma (LIP). The generated plasma behaves like a source ...

Laser ablation of a near-Earth object (NEO) on a collision course with Earth produces a cloud of ejecta that exerts a thrust on the NEO, deflecting it from its original trajectory. Ablation may be performed from afar by illuminating an Earth-targeting asteroid or comet with a stand-off “DE-STAR” system consisting of a large phased-array laser in Earth orbit. Alternatively, a much smaller stand...

low-intensity laser therapy (lilt) is defined to supply direct biostimulative light energy to the cells. while several studies have demonstrated that lilt has stimulating effects on bone cells and can accelerate the repair process of the bone, others reported delayed fracture healing or no effects after lilt. the aim of this article was to review the studies evaluating the biomodulation effects...

used in conjunction with or as a replacement for traditional methods, it is expected that specific laser technologies will become an essential component of contemporary dental practice over the next decade. the current review is designed to focus on the acid resistance of laser application and tries to introduce laser settings capable to achieve this feature for clinical application. applicatio...

OBJECTIVE To kill urothelial cancer cells while preserving healthy cells, this study used photothermal therapy (PTT). PTT techniques target urothelial cancer cells using gold nanoparticles (GNPs) and a green light laser. MATERIALS AND METHODS The GNPs were conjugated with anti-Mucin 7 antibodies, which acted as a probe for targeting tumor cells. Conjugated GNPs were exposed to a green light l...

PURPOSE To evaluate the safety, selectivity, and healing of retinal lesions created using a continuous line scanning laser. METHODS A 532-nm Nd:YAG laser (PASCAL) with retinal beam diameters of 40 μm and 66 μm was applied to 60 eyes of 30 Dutch-belted rabbits. Retinal exposure duration varied from 15 μs to 60 μs. Lesions were acutely assessed by ophthalmoscopy and fluorescein angiography. Ret...

plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. anthocyanin is affected by a variety of factors. light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. here, we have investigated the influence of the blue laser-diode (bld) and red laser-he ne (...