The interactions of calcium and neomycin with liposomes of various anionic phospholipids plus lecithin were studied by differential scanning calorimetry. Phosphatidylinositol bisphosphate differed from other acidic phospholipids in its interactions with both calcium and neomycin. Calcium, at concentrations as low as 1 mM, induced the appearance of a second transition peak in phosphatidylinosito...

We describe several modifications of the procedure originated by Gluck et al. [Amer. J. Obstet. Gynecol. 109, 440 (1971)] for evaluation of amniotic fluid lecithin/sphingomyelin (L/S) ratios. The acetone-precipitation step is eliminated, resulting in a faster extraction procedure and higher concentrations of phospholipids. For faster thin-layer chromatography, we use silica gel-impregnated shee...

The translational diffusion coefficient and the partition coefficient of a spin-labeled solute, di-t-butyl nitroxide, in an aqueous suspension of dipalmitoyl lecithin vesicles have been studied by electron spin resonance spectroscopy. When the lecithin is cooled through its phase transition temperature near 41 degrees C, some solute is "frozen out" of the bilayer, and the standard partial molar...

Familial lecithin-cholesterol acyltransferase deficiency is an uncommon autosomal recessive disorder from a heritable defect in esterification of plasma cholesterol. In 1968, the disease was described by Gjone and Norum in Norway. Our case was a 38-year-old woman. Her disease was manifested by presence of lower extremities edema, proteinuria, corneal opacities, increased plasma cholesterol, and...

Large and abrupt changes are observed at 38 degrees C in the 1100 cm(-1) region of the Raman spectrum of aqueous dipalmitoyl lecithin multilayers. They correspond to conformational changes due to the melting of the paraffin side chains. The addition of cholesterol to the multilayers broadens but does not abolish these changes. It is suggested that the addition of cholesterol decreases the inter...

This article introduces the formulation of alcohol-free, lecithin microemulsion-based gels (MBGs) prepared with gelatin as gelling agent. The influence of oil, water, lecithin and hydrophilic and lipophilic additives (linkers) on the rheological properties and appearance of these gels was systematically explored using ternary phase diagrams. Clear MBGs were obtained in regions of single phase m...

Milk thistle extract is a well-known hepatoprotectant with low bioavailability (20-50%). The objective of the present study is to prepare and characterize silymarin phytosomes and to test the hepatoprotective effect of the phytosomes in CCl4 induced liver injury in rats compared to milk thistle extract. Phytosomes were prepared using lecithin from soybeans and from egg yolk. The prepared phytos...

Pterostilbene, a dimethylether analog of resveratrol, has been found to have potent biological activity. However, the bioavailability pterostilbene in system is limited due its poor solubility an aqueous environment. A nanoemulsion was designed for this purpose. Lecithin-based formed after 3 cycles through high-pressure homogenizer at 500 psi. The rheological properties and particle size were m...

ABSTRACT Diets with high energy density and additives that enhance use are necessary for finishing feedlot cattle. The objective of the present study was to evaluate performance, ingestive behavior, apparent digestibility diet carcass traits steers fed concentrates from different sources, one derived starchy sources other, lipid combined or not whole soy lecithin, at a dose 40 g animal day-1. e...