Two-stcp polymerase ehain reaction (nested PCR) method was examined for the diagnosis of scrub typhus, Primers were derived from the type-spccific antigen (TSA) gene DNA sequences of Rickettsia tsutsugamptshi, GiLliam strain, These primers seryed to produce rickettsia-specific products in the amplification of template DNA prepared from all serovariants, G{11iam, Karp, Kato, Kawasaki, KLtroki an...